IRX4

Protein identityUniProt · HPA

UniProt accession

P78413

Gene

IRX4

Ensembl

ENSG00000113430

Chromosome

5

Canonical length

519 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Vesicles

OverviewNCBI Gene

Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in cell development; neuron differentiation; and regulation of DNA-templated transcription. Predicted to act upstream of or within heart development. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

519 residues, UniProt reviewed canonical sequence.

>P78413|IRX4
     1  MSYPQFGYPY SSAPQFLMAT NSLSTCCESG GRTLADSGPA ASAQAPVYCP VYESRLLATA
    61  RHELNSAAAL GVYGGPYGGS QGYGNYVTYG SEASAFYSLN SFDSKDGSGS AHGGLAPAAA
   121  AYYPYEPALG QYPYDRYGTM DSGTRRKNAT RETTSTLKAW LQEHRKNPYP TKGEKIMLAI
   181  ITKMTLTQVS TWFANARRRL KKENKMTWPP RNKCADEKRP YAEGEEEEGG EEEAREEPLK
   241  SSKNAEPVGK EEKELELSDL DDFDPLEAEP PACELKPPFH SLDGGLERVP AAPDGPVKEA
   301  SGALRMSLAA GGGAALDEDL ERARSCLRSA AAGPEPLPGA EGGPQVCEAK LGFVPAGASA
   361  GLEAKPRIWS LAHTATAAAA AATSLSQTEF PSCMLKRQGP AAPAAVSSAP ATSPSVALPH
   421  SGALDRHQDS PVTSLRNWVD GVFHDPILRH STLNQAWATA KGALLDPGPL GRSLGAGANV
   481  LTAPLARAFP PAVPQDAPAA GAARELLALP KAGGKPFCA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against IRX4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.67

Highest tissue expression

20 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about IRX4.

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 126 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Ventricular septal defect 1
• See cases

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.73

gnomAD pLI

0.01

gnomAD missense Z

-0.2

DepMap mean gene effect

0.14

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell development
• heart development
• negative regulation of transcription by RNA polymerase II
• neuron differentiation
• positive regulation of DNA-templated transcription
• regulation of transcription by RNA polymerase II
• establishment of animal organ orientation

Molecular functions

• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding

Cellular components

• chromatin
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Homeodomain
• Iroquois-class homeodomain protein
• KN homeodomain
• Homedomain-like superfamily
• Homeobox, conserved site
• Homeobox KN domain

KeywordsUniProt

• DNA-binding
• Homeobox
• Nucleus

InteractionsUniProt · HPA

Protein binding partners of IRX4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• VDR