IFI27

Protein identityUniProt · HPA

UniProt accession

P40305

Gene

IFI27

Ensembl

ENSG00000165949

Chromosome

14

Canonical length

122 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

Enables several functions, including RNA polymerase II-specific DNA-binding transcription factor binding activity; identical protein binding activity; and lamin binding activity. Involved in several processes, including defense response to other organism; protein K48-linked ubiquitination; and pyroptotic inflammatory response. Acts upstream of or within negative regulation of transcription by RNA polymerase II and regulation of protein export from nucleus. Located in mitochondrial membrane and nuclear inner membrane. Is active in mitochondrion. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

122 residues, UniProt reviewed canonical sequence.

>P40305|IFI27
     1  MEASALTSSA VTSVAKVVRV ASGSAVVLPL ARIATVVIGG VVAMAAVPMV LSAMGFTAAG
    61  IASSSIAAKM MSAAAIANGG GVASGSLVAT LQSLGATGLS GLTKFILGSI GSAIAAVIAR
   121  FY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against IFI27 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

3

Mean surface accessibility (rSASA)

0.48

Highest tissue expression

750 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.74

gnomAD pLI

0.01

gnomAD missense Z

0.41

DepMap mean gene effect

0.14

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic process
• defense response to virus
• extrinsic apoptotic signaling pathway
• host-mediated perturbation of viral genome replication
• innate immune response
• intrinsic apoptotic signaling pathway
• negative regulation of transcription by RNA polymerase II
• proteasome-mediated ubiquitin-dependent protein catabolic process
• protein K48-linked ubiquitination
• pyroptotic inflammatory response
• regulation of protein export from nucleus
• type I interferon-mediated signaling pathway

Molecular functions

• identical protein binding
• lamin binding
• molecular adaptor activity
• RNA polymerase II-specific DNA-binding transcription factor binding

Cellular components

• endoplasmic reticulum membrane
• mitochondrial membrane
• mitochondrial outer membrane
• mitochondrion
• nuclear envelope
• nuclear inner membrane

Protein domainsUniProt · Pfam · InterPro

• Interferon alpha-inducible protein IFI6/IFI27-like
• IFI6/IFI27-like domain superfamily
• Interferon-induced 6-16 family

KeywordsUniProt

• Antiviral defense
• Apoptosis
• Endoplasmic reticulum
• Host-virus interaction
• Immunity
• Innate immunity
• Isopeptide bond
• Membrane
• Mitochondrion
• Nucleus
• Transcription
• Transcription regulation
• Transit peptide
• Transmembrane
• Transmembrane helix
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of IFI27 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NR4A1
• SKP2