Seroatlas · Human Serome Atlas

HSF5

Heat shock factor protein 5

Also known as: FLJ40311, HSF5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q4G112
Gene: HSF5
Ensembl: ENSG00000176160
Chromosome: 17
Canonical length: 596 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm
Quaternary structure: Homooligomer

OverviewNCBI Gene

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in several processes, including male meiotic nuclear division; regulation of DNA-templated transcription; and spermatogenesis. Predicted to be located in XY body and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

596 residues, UniProt reviewed canonical sequence.

>Q4G112|HSF5
     1  MEALLSTPIN PNNFPAKLWR LVNSPRYRSI RWDGRGEGLL IDQPLFEAEL LSPPGPGGGG
    61  GTAGAGAEPE LFKTTSFTSF IRQLNLYGFR KVVLGGPGGG KPAGNGPLHH FHNPHFRRDQ
   121  PQLLVHLKRL TSANKAKLAA GLEVPCRPPN RFQRLLITSA SAATAPLQHQ QPPPPAGPRP
   181  EPHGPVAVGQ FHRSFRRDSL SPYSCVSTPS HDHSTYPLKG LDRTPVPHRI WQNSLGMHPG
   241  QVETSPTFSD KGVPFPVLQR FPTEVTYTLQ PSTTSVHVQQ GPQTMVSSSQ KYSNYTPSAQ
   301  YSQAYYPTAV LQCCSPTHMD ALSSCVTPTA SSYAHCNYFQ NPSMQSSYPV EFLPSNWPCS
   361  TTDENTKTEV NLEAVFQIVD ELHSSPKLEM VKVEPVENQC PTSPSYRGQH ILANSNNSNP
   421  CSASQASQLE PLTPVGSDIM SFVVGTEQAV ACSLPQSPEY IYTIHTAQPV ENSTIQESAA
   481  IQQAHVKLKE HLNHNPSPSS VVFVQEGPPF STHQVDANIK CQTSSRENIL PSEQMGFLIS
   541  EMGPASKPSE DTGLATPARY REHRSNSQQG KSPDLHLLVD VACKQERFPK EEELKE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HSF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.64
Highest tissue expression: 46 nTPM

Expression across tissuesHPA

Tissue

testis: 46 nTPM
lymph node: 1.1 nTPM
thymus: 0.8 nTPM
tonsil: 0.8 nTPM
appendix: 0.4 nTPM
bone marrow: 0.4 nTPM

Single-cell type

late primary spermatocytes: 135 nCPM
early primary spermatocytes: 50 nCPM
early spermatids: 36 nCPM
retinal ganglion cells: 27 nCPM
pdcs: 27 nCPM
hematopoietic stem cells: 25 nCPM

Immune cell

naive B-cell: 1.3 nTPM
memory B-cell: 1.1 nTPM
naive CD4 T-cell: 1.1 nTPM
T-reg: 0.9 nTPM
naive CD8 T-cell: 0.8 nTPM
memory CD4 T-cell: 0.6 nTPM

Brain region

cerebellum: 0.9 nTPM
pons: 0.5 nTPM
cerebral cortex: 0.4 nTPM
medulla oblongata: 0.4 nTPM
white matter: 0.4 nTPM
amygdala: 0.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.3
gnomAD pLI: 0.98
gnomAD missense Z: 2.6
DepMap mean gene effect: -0.19
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HSF5 as an antibody target. Whether an autoantibody or antibody against HSF5 could matter depends on whether native HSF5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HSF5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HSF5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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