Seroatlas · Human Serome Atlas

HS3ST3B1

Heparan sulfate glucosamine 3-O-sulfotransferase 3B1

Also known as: 30ST3B1, 3OST3B1, HS3SB_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y662
Gene
HS3ST3B1
Ensembl
ENSG00000125430
Chromosome
17
Canonical length
390 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Vesicles

OverviewNCBI Gene

The protein encoded by this gene is a type II integral membrane protein that belongs to the 3-O-sulfotransferases family. These proteins catalyze the addition of sulfate groups at the 3-OH position of glucosamine in heparan sulfate. The substrate specificity of individual members of the family is based on prior modification of the heparan sulfate chain, thus allowing different members of the family to generate binding sites for different proteins on the same heparan sulfate chain. Following treatment with a histone deacetylase inhibitor, expression of this gene is activated in a pancreatic cell line. The increased expression results in promotion of the epithelial-mesenchymal transition. In addition, the modification catalyzed by this protein allows herpes simplex virus membrane fusion and penetration. A very closely related homolog with an almost identical sulfotransferase domain maps less than 1 Mb away. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Canonical amino-acid sequenceUniProt

390 residues, UniProt reviewed canonical sequence.

>Q9Y662|HS3ST3B1
     1  MGQRLSGGRS CLDVPGRLLP QPPPPPPPVR RKLALLFAML CVWLYMFLYS CAGSCAAAPG
    61  LLLLGSGSRA AHDPPALATA PDGTPPRLPF RAPPATPLAS GKEMAEGAAS PEEQSPEVPD
   121  SPSPISSFFS GSGSKQLPQA IIIGVKKGGT RALLEFLRVH PDVRAVGAEP HFFDRSYDKG
   181  LAWYRDLMPR TLDGQITMEK TPSYFVTREA PARISAMSKD TKLIVVVRDP VTRAISDYTQ
   241  TLSKRPDIPT FESLTFKNRT AGLIDTSWSA IQIGIYAKHL EHWLRHFPIR QMLFVSGERL
   301  ISDPAGELGR VQDFLGLKRI ITDKHFYFNK TKGFPCLKKA EGSSRPHCLG KTKGRTHPEI
   361  DREVVRRLRE FYRPFNLKFY QMTGHDFGWD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HS3ST3B1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
27 nTPM

Expression across tissuesHPA

Tissue

  • liver: 27 nTPM
  • retina: 11 nTPM
  • lymph node: 5.9 nTPM
  • salivary gland: 5 nTPM
  • tonsil: 4.5 nTPM
  • appendix: 4.4 nTPM

Single-cell type

  • neutrophils: 331 nCPM
  • pdcs: 178 nCPM
  • cone photoreceptor cells: 172 nCPM
  • neutrophil progenitors: 115 nCPM
  • podocytes: 109 nCPM
  • lacrimal acinar cells: 106 nCPM

Immune cell

  • plasmacytoid DC: 35 nTPM
  • T-reg: 26 nTPM
  • naive CD4 T-cell: 12 nTPM
  • memory CD4 T-cell: 12 nTPM
  • naive CD8 T-cell: 12 nTPM
  • gdT-cell: 10 nTPM

Brain region

  • medulla oblongata: 12 nTPM
  • spinal cord: 6.9 nTPM
  • midbrain: 5.6 nTPM
  • pons: 4.9 nTPM
  • choroid plexus: 3.9 nTPM
  • thalamus: 3.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.78
gnomAD pLI
0.29
gnomAD missense Z
0.46
DepMap mean gene effect
-0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HS3ST3B1 as an antibody target. Whether an autoantibody or antibody against HS3ST3B1 could matter depends on whether native HS3ST3B1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HS3ST3B1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HS3ST3B1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HS3ST3B1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...