Seroatlas · Human Serome Atlas

HS3ST1

Heparan sulfate glucosamine 3-O-sulfotransferase 1

Also known as: 3OST1, HS3S1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O14792
Gene
HS3ST1
Ensembl
ENSG00000002587
Chromosome
4
Canonical length
307 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Vesicles
Secretome location
Intracellular and membrane

OverviewNCBI Gene

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

307 residues, UniProt reviewed canonical sequence.

>O14792|HS3ST1
     1  MAALLLGAVL LVAQPQLVPS RPAELGQQEL LRKAGTLQDD VRDGVAPNGS AQQLPQTIII
    61  GVRKGGTRAL LEMLSLHPDV AAAENEVHFF DWEEHYSHGL GWYLSQMPFS WPHQLTVEKT
   121  PAYFTSPKVP ERVYSMNPSI RLLLILRDPS ERVLSDYTQV FYNHMQKHKP YPSIEEFLVR
   181  DGRLNVDYKA LNRSLYHVHM QNWLRFFPLR HIHIVDGDRL IRDPFPEIQK VERFLKLSPQ
   241  INASNFYFNK TKGFYCLRDS GRDRCLHESK GRAHPQVDPK LLNKLHEYFH EPNKKFFELV
   301  GRTFDWH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HS3ST1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 29 nTPM
  • urinary bladder: 14 nTPM
  • cerebellum: 13 nTPM
  • gallbladder: 12 nTPM
  • esophagus: 11 nTPM
  • stomach: 6.1 nTPM

Single-cell type

  • urothelial cells: 544 nCPM
  • esophageal apical cells: 508 nCPM
  • ocular epithelial cells: 413 nCPM
  • conjunctival goblet cells: 379 nCPM
  • respiratory secretory cells: 272 nCPM
  • ovarian stromal cells: 245 nCPM

Immune cell

  • plasmacytoid DC: 43 nTPM
  • naive B-cell: 27 nTPM
  • memory B-cell: 15 nTPM
  • total PBMC: 1.1 nTPM
  • myeloid DC: 0.7 nTPM
  • intermediate monocyte: 0.3 nTPM

Brain region

  • cerebellum: 59 nTPM
  • cerebral cortex: 20 nTPM
  • white matter: 19 nTPM
  • thalamus: 19 nTPM
  • hypothalamus: 16 nTPM
  • medulla oblongata: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.11
gnomAD pLI
0
gnomAD missense Z
1.09
DepMap mean gene effect
0.08
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HS3ST1 as an antibody target. Whether an autoantibody or antibody against HS3ST1 could matter depends on whether native HS3ST1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HS3ST1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HS3ST1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HS3ST1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...