HLA-DRB5

Protein identityUniProt · HPA

UniProt accession

Q30154

Gene

HLA-DRB5

Ensembl

ENSG00000198502

Chromosome

6

Canonical length

266 aa

Protein class

Human disease related genes, Predicted membrane proteins

Quaternary structure

Homotrimer

OverviewNCBI Gene

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene. [provided by RefSeq, Feb 2020]

Canonical amino-acid sequenceUniProt

266 residues, UniProt reviewed canonical sequence.

>Q30154|HLA-DRB5
     1  MVCLKLPGGS YMAKLTVTLM VLSSPLALAG DTRPRFLQQD KYECHFFNGT ERVRFLHRDI
    61  YNQEEDLRFD SDVGEYRAVT ELGRPDAEYW NSQKDFLEDR RAAVDTYCRH NYGVGESFTV
   121  QRRVEPKVTV YPARTQTLQH HNLLVCSVNG FYPGSIEVRW FRNSQEEKAG VVSTGLIQNG
   181  DWTFQTLVML ETVPRSGEVY TCQVEHPSVT SPLTVEWRAQ SESAQSKMLS GVGGFVLGLL
   241  FLGAGLFIYF KNQKGHSGLH PTGLVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HLA-DRB5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

397 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about HLA-DRB5.

Disease | ImmuneIEDB

Conditions an epitope on HLA-DRB5 was assayed in.

• multiple sclerosis T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.85

gnomAD pLI

0

gnomAD missense Z

0.61

DepMap mean gene effect

0.13

DepMap dependency class

none

OntologyGO

Biological processes

• adaptive immune response
• antigen processing and presentation of exogenous peptide antigen via MHC class II
• peptide antigen assembly with MHC class II protein complex
• positive regulation of immune response
• positive regulation of T cell activation

Molecular functions

• MHC class II protein complex binding
• peptide antigen binding

Cellular components

• clathrin-coated endocytic vesicle membrane
• endocytic vesicle membrane
• ER to Golgi transport vesicle membrane
• extracellular exosome
• Golgi membrane
• late endosome membrane
• lumenal side of endoplasmic reticulum membrane
• lysosomal membrane
• MHC class II protein complex
• plasma membrane
• trans-Golgi network membrane
• transport vesicle membrane

Protein domainsUniProt · Pfam · InterPro

• MHC class II, beta chain, N-terminal
• Immunoglobulin/major histocompatibility complex, conserved site
• Immunoglobulin C1-set
• Immunoglobulin-like domain
• MHC classes I/II-like antigen recognition protein
• Immunoglobulin-like fold
• MHC class II, alpha/beta chain, N-terminal
• Immunoglobulin-like domain superfamily
• Major Histocompatibility Complex/Immunoglobulin
• Class II histocompatibility antigen, beta domain
• Immunoglobulin C1-set domain

KeywordsUniProt

• Adaptive immunity
• Cell membrane
• Disulfide bond
• Endoplasmic reticulum
• Endosome
• Glycoprotein
• Golgi apparatus
• Immunity
• Lysosome
• Membrane
• MHC II
• Signal
• Transmembrane
• Transmembrane helix
• Ubl conjugation