HLA-DOB

Protein identityUniProt · HPA

UniProt accession

P13765

Gene

HLA-DOB

Ensembl

ENSG00000241106

Chromosome

6

Canonical length

273 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

273 residues, UniProt reviewed canonical sequence.

>P13765|HLA-DOB
     1  MGSGWVPWVV ALLVNLTRLD SSMTQGTDSP EDFVIQAKAD CYFTNGTEKV QFVVRFIFNL
    61  EEYVRFDSDV GMFVALTKLG QPDAEQWNSR LDLLERSRQA VDGVCRHNYR LGAPFTVGRK
   121  VQPEVTVYPE RTPLLHQHNL LHCSVTGFYP GDIKIKWFLN GQEERAGVMS TGPIRNGDWT
   181  FQTVVMLEMT PELGHVYTCL VDHSSLLSPV SVEWRAQSEY SWRKMLSGIA AFLLGLIFLL
   241  VGIVIQLRAQ KGYVRTQMSG NEVSRAVLLP QSC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HLA-DOB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

41 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.35

gnomAD pLI

0

gnomAD missense Z

0.18

DepMap mean gene effect

-0.1

DepMap dependency class

selective

OntologyGO

Biological processes

• adaptive immune response
• antigen processing and presentation of exogenous peptide antigen via MHC class II
• negative regulation of antigen processing and presentation of peptide antigen via MHC class II
• peptide antigen assembly with MHC class II protein complex
• positive regulation of immune response
• positive regulation of T cell activation

Molecular functions

• MHC class II protein complex binding
• MHC class II receptor activity
• peptide antigen binding

Cellular components

• late endosome membrane
• lysosomal membrane
• lysosome
• MHC class II protein complex

Protein domainsUniProt · Pfam · InterPro

• MHC class II, beta chain, N-terminal
• Immunoglobulin/major histocompatibility complex, conserved site
• Immunoglobulin C1-set
• Immunoglobulin-like domain
• MHC classes I/II-like antigen recognition protein
• Immunoglobulin-like fold
• MHC class II, alpha/beta chain, N-terminal
• Immunoglobulin-like domain superfamily
• Major Histocompatibility Complex/Immunoglobulin
• Class II histocompatibility antigen, beta domain
• Immunoglobulin C1-set domain

KeywordsUniProt

• Adaptive immunity
• Disulfide bond
• Endosome
• Glycoprotein
• Immunity
• Lysosome
• Membrane
• MHC II
• Signal
• Transmembrane
• Transmembrane helix