HLA-DOA

Protein identityUniProt · HPA

UniProt accession

P06340

Gene

HLA-DOA

Ensembl

ENSG00000204252

Chromosome

6

Canonical length

250 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

250 residues, UniProt reviewed canonical sequence.

>P06340|HLA-DOA
     1  MALRAGLVLG FHTLMTLLSP QEAGATKADH MGSYGPAFYQ SYGASGQFTH EFDEEQLFSV
    61  DLKKSEAVWR LPEFGDFARF DPQGGLAGIA AIKAHLDILV ERSNRSRAIN VPPRVTVLPK
   121  SRVELGQPNI LICIVDNIFP PVINITWLRN GQTVTEGVAQ TSFYSQPDHL FRKFHYLPFV
   181  PSAEDVYDCQ VEHWGLDAPL LRHWELQVPI PPPDAMETLV CALGLAIGLV GFLVGTVLII
   241  MGTYVSSVPR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HLA-DOA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

27 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about HLA-DOA.

Disease | ImmuneIEDB

Conditions an epitope on HLA-DOA was assayed in.

• skin melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.85

gnomAD pLI

0

gnomAD missense Z

0.47

DepMap mean gene effect

-0.01

DepMap dependency class

none

OntologyGO

Biological processes

• adaptive immune response
• antigen processing and presentation of exogenous peptide antigen via MHC class II
• negative regulation of antigen processing and presentation of peptide antigen via MHC class II
• peptide antigen assembly with MHC class II protein complex
• positive regulation of immune response
• positive regulation of T cell activation
• regulation of T cell differentiation

Molecular functions

• MHC class II protein complex binding
• MHC class II receptor activity
• peptide antigen binding

Cellular components

• late endosome membrane
• lysosomal membrane
• MHC class II protein complex
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• MHC class II, alpha chain, N-terminal
• Immunoglobulin/major histocompatibility complex, conserved site
• Immunoglobulin C1-set
• Immunoglobulin-like domain
• MHC classes I/II-like antigen recognition protein
• Immunoglobulin-like fold
• MHC class II, alpha/beta chain, N-terminal
• Immunoglobulin-like domain superfamily
• Major Histocompatibility Complex/Immunoglobulin
• Class II histocompatibility antigen, alpha domain
• Immunoglobulin C1-set domain

KeywordsUniProt

• Adaptive immunity
• Disulfide bond
• Endosome
• Glycoprotein
• Immunity
• Lysosome
• Membrane
• MHC II
• Signal
• Transmembrane
• Transmembrane helix