HLA-DMB

Protein identityUniProt · HPA

UniProt accession

P28068

Gene

HLA-DMB

Ensembl

ENSG00000242574

Chromosome

6

Canonical length

263 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Vesicles

OverviewNCBI Gene

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

263 residues, UniProt reviewed canonical sequence.

>P28068|HLA-DMB
     1  MITFLPLLLG LSLGCTGAGG FVAHVESTCL LDDAGTPKDF TYCISFNKDL LTCWDPEENK
    61  MAPCEFGVLN SLANVLSQHL NQKDTLMQRL RNGLQNCATH TQPFWGSLTN RTRPPSVQVA
   121  KTTPFNTREP VMLACYVWGF YPAEVTITWR KNGKLVMPHS SAHKTAQPNG DWTYQTLSHL
   181  ALTPSYGDTY TCVVEHTGAP EPILRDWTPG LSPMQTLKVS VSAVTLGLGL IIFSLGVISW
   241  RRAGHSSYTP LPGSNYSEGW HIS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HLA-DMB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

101 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.98

gnomAD pLI

0

gnomAD missense Z

1.59

DepMap mean gene effect

0.06

DepMap dependency class

none

OntologyGO

Biological processes

• adaptive immune response
• antigen processing and presentation of exogenous peptide antigen via MHC class II
• peptide antigen assembly with MHC class II protein complex
• positive regulation of immune response
• positive regulation of T cell activation
• positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
• positive regulation of T cell proliferation
• MHC class II protein complex assembly

Molecular functions

• MHC class II protein complex binding
• peptide antigen binding

Cellular components

• late endosome membrane
• lysosomal membrane
• MHC class II protein complex

Protein domainsUniProt · Pfam · InterPro

• MHC class II, beta chain, N-terminal
• Immunoglobulin/major histocompatibility complex, conserved site
• Immunoglobulin C1-set
• Immunoglobulin-like domain
• MHC classes I/II-like antigen recognition protein
• Immunoglobulin-like fold
• MHC class II, alpha/beta chain, N-terminal
• Immunoglobulin-like domain superfamily
• Major Histocompatibility Complex/Immunoglobulin
• Class II histocompatibility antigen, beta domain
• Immunoglobulin C1-set domain

KeywordsUniProt

• Adaptive immunity
• Disulfide bond
• Endosome
• Glycoprotein
• Immunity
• Immunoglobulin domain
• Lysosome
• Membrane
• MHC II
• Signal
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of HLA-DMB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MHCII
• HLA-DRA