Seroatlas · Human Serome Atlas

HES4

Transcription factor HES-4

Also known as: bHLHb42, HES4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HCC6
Gene: HES4
Ensembl: ENSG00000188290
Chromosome: 1
Canonical length: 221 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in cell differentiation; nervous system development; and regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

221 residues, UniProt reviewed canonical sequence.

>Q9HCC6|HES4
     1  MAADTPGKPS ASPMAGAPAS ASRTPDKPRS AAEHRKSSKP VMEKRRRARI NESLAQLKTL
    61  ILDALRKESS RHSKLEKADI LEMTVRHLRS LRRVQVTAAL SADPAVLGKY RAGFHECLAE
   121  VNRFLAGCEG VPADVRSRLL GHLAACLRQL GPSRRPASLS PAAPAEAPAP EVYAGRPLLP
   181  SLGGPFPLLA PPLLPGLTRA LPAAPRAGPQ GPGGPWRPWL R

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HES4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.58
Highest tissue expression: 31 nTPM

Expression across tissuesHPA

Tissue

blood vessel: 31 nTPM
basal ganglia: 24 nTPM
heart muscle: 23 nTPM
hypothalamus: 17 nTPM
cerebral cortex: 15 nTPM
amygdala: 14 nTPM

Single-cell type

vascular smooth muscle cells: 647 nCPM
urothelial cells: 483 nCPM
fallopian secretory cells: 434 nCPM
epididymal efferent duct absorptive cells: 416 nCPM
pericytes: 356 nCPM
respiratory secretory cells: 290 nCPM

Immune cell

eosinophil: 13 nTPM
non-classical monocyte: 1.3 nTPM
intermediate monocyte: 1 nTPM
myeloid DC: 0.3 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM

Brain region

cerebral cortex: 90 nTPM
thalamus: 64 nTPM
medulla oblongata: 60 nTPM
hypothalamus: 51 nTPM
pons: 50 nTPM
midbrain: 47 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.95
gnomAD pLI: 0
gnomAD missense Z: -0.47
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HES4 as an antibody target. Whether an autoantibody or antibody against HES4 could matter depends on whether native HES4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HES4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HES4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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