Seroatlas · Human Serome Atlas

HES3

Transcription factor HES-3

Also known as: bHLHb43, HES3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5TGS1
Gene: HES3
Ensembl: ENSG00000173673
Chromosome: 1
Canonical length: 186 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of neurogenesis. Predicted to act upstream of or within several processes, including nervous system development; regulation of timing of neuron differentiation; and regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

186 residues, UniProt reviewed canonical sequence.

>Q5TGS1|HES3
     1  MEKKRRARIN VSLEQLKSLL EKHYSHQIRK RKLEKADILE LSVKYMRSLQ NSLQGLWPVP
    61  RGAEQPSGFR SCLPGVSQLL RRGDEVGSGL RCPLVPESAA GSTMDSAGLG QEAPALFRPC
   121  TPAVWAPAPA AGGPRSPPPL LLLPESLPGS SASVPPPQPA SSRCAESPGL GLRVWRPWGS
   181  PGDDLN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HES3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.64
Highest tissue expression: 0.7 nTPM

Expression across tissuesHPA

Tissue

thymus: 0.7 nTPM
testis: 0.1 nTPM
adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM
appendix: 0 nTPM

Single-cell type

retinal amacrine cells: 0.7 nCPM
early spermatids: 0.1 nCPM
endometrial stromal cells: 0.1 nCPM
undifferentiated spermatogonia: 0.1 nCPM
adipocytes: 0 nCPM
adrenal cortex cells: 0 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebral cortex: 1 nTPM
amygdala: 0.7 nTPM
thalamus: 0.7 nTPM
hypothalamus: 0.6 nTPM
midbrain: 0.6 nTPM
basal ganglia: 0.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.25
gnomAD pLI: 0.28
gnomAD missense Z: 0.58
DepMap mean gene effect: -0.15
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 1% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HES3 as an antibody target. Whether an autoantibody or antibody against HES3 could matter depends on whether native HES3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HES3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HES3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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