GUCY2F

Protein identityUniProt · HPA

UniProt accession

P51841

Gene

GUCY2F

Ensembl

ENSG00000101890

Chromosome

X

Canonical length

1108 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted membrane proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a guanylyl cyclase found predominantly in photoreceptors in the retina. The encoded protein is thought to be involved in resynthesis of cGMP after light activation of the visual signal transduction cascade, allowing a return to the dark state. This protein is a single-pass type I membrane protein. Defects in this gene may be a cause of X-linked retinitis pigmentosa. [provided by RefSeq, Dec 2008]

Canonical amino-acid sequenceUniProt

1108 residues, UniProt reviewed canonical sequence.

>P51841|GUCY2F
     1  MFLGLGRFSR LVLWFAAFRK LLGHHGLASA KFLWCLCLLS VMSLPQQVWT LPYKIGVVGP
    61  WACDSLFSKA LPEVAARLAI ERINRDPSFD LSYSFEYVIL NEDCQTSRAL SSFISHHQMA
   121  SGFIGPTNPG YCEAASLLGN SWDKGIFSWA CVNYELDNKI SYPTFSRTLP SPIRVLVTVM
   181  KYFQWAHAGV ISSDEDIWVH TANRVASALR SHGLPVGVVL TTGQDSQSMR KALQRIHQAD
   241  RIRIIIMCMH SALIGGETQM HLLECAHDLK MTDGTYVFVP YDALLYSLPY KHTPYRVLRN
   301  NPKLREAYDA VLTITVESQE KTFYQAFTEA AARGEIPEKL EFDQVSPLFG TIYNSIYFIA
   361  QAMNNAMKEN GQAGAASLVQ HSRNMQFHGF NQLMRTDSNG NGISEYVILD TNLKEWELHS
   421  TYTVDMEMEL LRFGGTPIHF PGGRPPRADA KCWFAEGKIC HGGIDPAFAM MVCLTLLIAL
   481  LSINGFAYFI RRRINKIQLI KGPNRILLTL EDVTFINPHF GSKRGSRASV SFQITSEVQS
   541  GRSPRLSFSS GSLTPATYEN SNIAIYEGDW VWLKKFSLGD FGDLKSIKSR ASDVFEMMKD
   601  LRHENINPLL GFFYDSGMFA IVTEFCSRGS LEDILTNQDV KLDWMFKSSL LLDLIKGMKY
   661  LHHREFVHGR LKSRNCVVDG RFVLKVTDYG FNDILEMLRL SEEESSMEEL LWTAPELLRA
   721  PRGSRLGSFA GDVYSFAIIM QEVMVRGTPF CMMDLPAQEI INRLKKPPPV YRPVVPPEHA
   781  PPECLQLMKQ CWAEAAEQRP TFDEIFNQFK TFNKGKKTNI IDSMLRMLEQ YSSNLEDLIR
   841  ERTEELEIEK QKTEKLLTQM LPPSVAESLK KGCTVEPEGF DLVTLYFSDI VGFTTISAMS
   901  EPIEVVDLLN DLYTLFDAII GSHDVYKVET IGDAYMVASG LPKRNGSRHA AEIANMSLDI
   961  LSSVGTFKMR HMPEVPVRIR IGLHSGPVVA GVVGLTMPRY CLFGDTVNTA SRMESTGLPY
  1021  RIHVSLSTVT ILQNLSEGYE VELRGRTELK GKGTEETFWL IGKKGFMKPL PVPPPVDKDG
  1081  QVGHGLQPVE IAAFQRRKAE RQLVRNKP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GUCY2F can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

39 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.97

gnomAD pLI

0

gnomAD missense Z

-0.21

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cGMP biosynthetic process
• cGMP-mediated signaling
• detection of light stimulus involved in visual perception
• receptor guanylyl cyclase signaling pathway
• regulation of opsin-mediated signaling pathway
• visual perception

Molecular functions

• ATP binding
• GTP binding
• guanylate cyclase activity
• identical protein binding
• peptide receptor activity
• protein kinase activity
• protein-containing complex binding
• signaling receptor activity

Cellular components

• nuclear outer membrane
• photoreceptor disc membrane
• plasma membrane
• rod photoreceptor outer segment

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Adenylyl cyclase class-3/4/guanylyl cyclase
• Serine-threonine/tyrosine-protein kinase, catalytic domain
• Receptor, ligand binding region
• Protein kinase-like domain superfamily
• Haem NO binding associated
• Adenylyl cyclase class-4/guanylyl cyclase, conserved site
• Periplasmic binding protein-like I
• Nucleotide cyclase
• Cyclic nucleotide synthase
• Adenylate and Guanylate cyclase catalytic domain
• Receptor family ligand binding region
• Heme NO binding associated
• Protein tyrosine and serine/threonine kinase

KeywordsUniProt

• Cell projection
• cGMP biosynthesis
• Disulfide bond
• GTP-binding
• Lyase
• Membrane
• Nucleotide-binding
• Sensory transduction
• Signal
• Transmembrane
• Transmembrane helix
• Vision

InteractionsUniProt · HPA

Protein binding partners of GUCY2F in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RD3