GSTA3

Protein identityUniProt · HPA

UniProt accession

Q16772

Gene

GSTA3

Ensembl

ENSG00000174156

Chromosome

6

Canonical length

222 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

222 residues, UniProt reviewed canonical sequence.

>Q16772|GSTA3
     1  MAGKPKLHYF NGRGRMEPIR WLLAAAGVEF EEKFIGSAED LGKLRNDGSL MFQQVPMVEI
    61  DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYTEGMA DLNEMILLLP LCRPEEKDAK
   121  IALIKEKTKS RYFPAFEKVL QSHGQDYLVG NKLSRADISL VELLYYVEEL DSSLISNFPL
   181  LKALKTRISN LPTVKKFLQP GSPRKPPADA KALEEARKIF RF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GSTA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

62 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.12

gnomAD pLI

0

gnomAD missense Z

-0.42

DepMap mean gene effect

-0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• glutathione metabolic process
• lipid metabolic process
• xenobiotic metabolic process

Molecular functions

• glutathione transferase activity

Cellular components

• cytosol
• extracellular exosome

Protein domainsUniProt · Pfam · InterPro

• Glutathione S-transferase, alpha class
• Glutathione S-transferase, N-terminal
• Glutathione S-transferase, C-terminal
• Glutathione S-transferase, C-terminal-like
• Thioredoxin-like superfamily
• Glutathione S-transferase, C-terminal domain superfamily
• Glutathione transferase family
• Glutathione S-transferase superfamily
• Glutathione S-transferase, C-terminal domain
• Glutathione S-transferase, N-terminal domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Lipid metabolism
• Transferase