GPX2

Protein identityUniProt · HPA

UniProt accession

P18283

Gene

GPX2

Ensembl

ENSG00000176153

Chromosome

14

Canonical length

190 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Cytokinetic bridge,Mitotic spindle,Cytosol

Quaternary structure

Homotetramer

OverviewNCBI Gene

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

Canonical amino-acid sequenceUniProt

190 residues, UniProt reviewed canonical sequence.

>P18283|GPX2
     1  MAFIAKSFYD LSAISLDGEK VDFNTFRGRA VLIENVASLU GTTTRDFTQL NELQCRFPRR
    61  LVVLGFPCNQ FGHQENCQNE EILNSLKYVR PGGGYQPTFT LVQKCEVNGQ NEHPVFAYLK
   121  DKLPYPYDDP FSLMTDPKLI IWSPVRRSDV AWNFEKFLIG PEGEPFRRYS RTFPTINIEP
   181  DIKRLLKVAI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GPX2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0

Highest tissue expression

616 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.69

gnomAD pLI

0

gnomAD missense Z

0.21

DepMap mean gene effect

0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• response to oxidative stress

Molecular functions

• electron transfer activity
• glutathione peroxidase activity
• phospholipid-hydroperoxide glutathione peroxidase activity

Cellular components

• cytoplasm
• cytosol
• intercellular bridge
• mitotic spindle

Protein domainsUniProt · Pfam · InterPro

• Glutathione peroxidase
• Glutathione peroxidase active site
• Glutathione peroxidase conserved site
• Thioredoxin-like superfamily
• Glutathione peroxidase

KeywordsUniProt

• Cytoplasm
• Oxidoreductase
• Peroxidase
• Selenocysteine