GMDS

Protein identityUniProt · HPA

UniProt accession

O60547

Gene

GMDS

Ensembl

ENSG00000112699

Chromosome

6

Canonical length

372 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).[supplied by OMIM, Aug 2009]

Canonical amino-acid sequenceUniProt

372 residues, UniProt reviewed canonical sequence.

>O60547|GMDS
     1  MAHAPARCPS ARGSGDGEMG KPRNVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF
    61  NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL
   121  AEYTADVDGV GTLRLLDAVK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG
   181  AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL
   241  GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFL HIGKTIVWEG
   301  KNENEVGRCK ETGKVHVTVD LKYYRPTEVD FLQGDCTKAK QKLNWKPRVA FDELVREMVH
   361  ADVELMRTNP NA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GMDS can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

100 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.27

gnomAD pLI

0.99

gnomAD missense Z

1.82

DepMap mean gene effect

0.16

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• 'de novo' GDP-L-fucose biosynthetic process
• GDP-L-fucose biosynthetic process
• GDP-mannose metabolic process
• Notch signaling pathway

Molecular functions

• identical protein binding
• NADP+ binding
• GDP-mannose 4,6-dehydratase activity

Cellular components

• cytoplasm
• cytosol
• extracellular exosome

Protein domainsUniProt · Pfam · InterPro

• NAD(P)-binding domain
• NAD(P)-binding domain superfamily
• GDP-mannose 4,6 dehydratase
• GDP-mannose 4,6-dehydratase

KeywordsUniProt

• Acetylation
• Lyase
• NADP
• Phosphoprotein