Seroatlas · Human Serome Atlas

GCNT3

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Also known as: C2/4GnT, C2GnT-M, C2GnT2, GCNT3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O95395
Gene
GCNT3
Ensembl
ENSG00000140297
Chromosome
15
Canonical length
438 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Golgi apparatus,Vesicles

OverviewNCBI Gene

This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]

Canonical amino-acid sequenceUniProt

438 residues, UniProt reviewed canonical sequence.

>O95395|GCNT3
     1  MVQWKRLCQL HYLWALGCYM LLATVALKLS FRLKCDSDHL GLESRESQSQ YCRNILYNFL
    61  KLPAKRSINC SGVTRGDQEA VLQAILNNLE VKKKREPFTD THYLSLTRDC EHFKAERKFI
   121  QFPLSKEEVE FPIAYSMVIH EKIENFERLL RAVYAPQNIY CVHVDEKSPE TFKEAVKAII
   181  SCFPNVFIAS KLVRVVYASW SRVQADLNCM EDLLQSSVPW KYFLNTCGTD FPIKSNAEMV
   241  QALKMLNGRN SMESEVPPKH KETRWKYHFE VVRDTLHLTN KKKDPPPYNL TMFTGNAYIV
   301  ASRDFVQHVL KNPKSQQLIE WVKDTYSPDE HLWATLQRAR WMPGSVPNHP KYDISDMTSI
   361  ARLVKWQGHE GDIDKGAPYA PCSGIHQRAI CVYGAGDLNW MLQNHHLLAN KFDPKVDDNA
   421  LQCLEEYLRY KAIYGTEL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GCNT3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
134 nTPM

Expression across tissuesHPA

Tissue

  • colon: 134 nTPM
  • small intestine: 116 nTPM
  • rectum: 116 nTPM
  • duodenum: 92 nTPM
  • gallbladder: 71 nTPM
  • salivary gland: 54 nTPM

Single-cell type

  • colonocytes: 269 nCPM
  • enterocytes: 180 nCPM
  • endometrial glandular cells: 163 nCPM
  • esophageal apical cells: 147 nCPM
  • conjunctival goblet cells: 133 nCPM
  • renal collecting duct principal cells: 127 nCPM

Immune cell

  • basophil: 0.8 nTPM
  • neutrophil: 0.6 nTPM
  • plasmacytoid DC: 0.3 nTPM
  • classical monocyte: 0.1 nTPM
  • eosinophil: 0.1 nTPM
  • gdT-cell: 0.1 nTPM

Brain region

  • cerebellum: 3.1 nTPM
  • midbrain: 2.9 nTPM
  • cerebral cortex: 2.6 nTPM
  • white matter: 2.5 nTPM
  • choroid plexus: 2.4 nTPM
  • hypothalamus: 2.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.43
gnomAD pLI
0
gnomAD missense Z
-1.65
DepMap mean gene effect
-0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GCNT3 as an antibody target. Whether an autoantibody or antibody against GCNT3 could matter depends on whether native GCNT3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GCNT3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GCNT3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/GCNT3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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