Seroatlas · Human Serome Atlas

GCFC2

Intron Large complex component GCFC2

Also known as: C2orf3, DNABF, GCF, GCFC2_HUMAN, TCF9

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P16383
Gene: GCFC2
Ensembl: ENSG00000005436
Chromosome: 2
Canonical length: 781 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

781 residues, UniProt reviewed canonical sequence.

>P16383|GCFC2
     1  MAHRPKRTFR QRAADSSDSD GAEESPAEPG APRELPVPGS AEEEPPSGGG RAQVAGLPHR
    61  VRGPRGRGRV WASSRRATKA APRADEGSES RTLDVSTDEE DKIHHSSESK DDQGLSSDSS
   121  SSLGEKELSS TVKIPDAAFI QAARRKRELA RAQDDYISLD VQHTSSISGM KRESEDDPES
   181  EPDDHEKRIP FTLRPQTLRQ RMAEESISRN EETSEESQED EKQDTWEQQQ MRKAVKIIEE
   241  RDIDLSCGNG SSKVKKFDTS ISFPPVNLEI IKKQLNTRLT LLQETHRSHL REYEKYVQDV
   301  KSSKSTIQNL ESSSNQALNC KFYKSMKIYV ENLIDCLNEK IINIQEIESS MHALLLKQAM
   361  TFMKRRQDEL KHESTYLQQL SRKDETSTSG NFSVDEKTQW ILEEIESRRT KRRQARVLSG
   421  NCNHQEGTSS DDELPSAEMI DFQKSQGDIL QKQKKVFEEV QDDFCNIQNI LLKFQQWREK
   481  FPDSYYEAFI SLCIPKLLNP LIRVQLIDWN PLKLESTGLK EMPWFKSVEE FMDSSVEDSK
   541  KESSSDKKVL SAIINKTIIP RLTDFVEFLW DPLSTSQTTS LITHCRVILE EHSTCENEVS
   601  KSRQDLLKSI VSRMKKAVED DVFIPLYPKS AVENKTSPHS KFQERQFWSG LKLFRNILLW
   661  NGLLTDDTLQ ELGLGKLLNR YLIIALLNAT PGPDVVKKCN QVAACLPEKW FENSAMRTSI
   721  PQLENFIQFL LQSAHKLSRS EFRDEVEEII LILVKIKALN QAESFIGEHH LDHLKSLIKE
   781  D

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GCFC2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 29 nTPM

Expression across tissuesHPA

Tissue

liver: 29 nTPM
skeletal muscle: 28 nTPM
cervix: 18 nTPM
kidney: 17 nTPM
skin: 15 nTPM
ovary: 14 nTPM

Single-cell type

myonuclei: 474 nCPM
cardiomyocytes: 217 nCPM
adipocytes: 146 nCPM
late spermatids: 134 nCPM
distal convoluted tubule cells: 121 nCPM
fibro-adipogenic progenitors: 119 nCPM

Immune cell

memory B-cell: 18 nTPM
naive CD4 T-cell: 14 nTPM
plasmacytoid DC: 13 nTPM
naive CD8 T-cell: 13 nTPM
naive B-cell: 13 nTPM
memory CD8 T-cell: 12 nTPM

Brain region

white matter: 29 nTPM
basal ganglia: 27 nTPM
spinal cord: 26 nTPM
midbrain: 25 nTPM
thalamus: 25 nTPM
cerebellum: 24 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.17
gnomAD pLI: 0
gnomAD missense Z: 0
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

DNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of GCFC2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GCFC2 as an antibody target. Whether an autoantibody or antibody against GCFC2 could matter depends on whether native GCFC2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GCFC2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GCFC2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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