Seroatlas · Human Serome Atlas

G2E3

G2/M phase-specific E3 ubiquitin-protein ligase

Also known as: FLJ20333, G2E3_HUMAN, KIAA1333, PHF7B

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q7L622
Gene: G2E3
Ensembl: ENSG00000092140
Chromosome: 14
Canonical length: 706 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Golgi apparatus,Vesicles,Cytosol

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in apoptotic process and protein ubiquitination. Predicted to act upstream of or within blastocyst development; negative regulation of intrinsic apoptotic signaling pathway; and protein polyubiquitination. Located in Golgi apparatus and cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

706 residues, UniProt reviewed canonical sequence.

>Q7L622|G2E3
     1  MNESKPGDSQ NLACVFCRKH DDCPNKYGEK KTKEKWNLTV HYYCLLMSSG IWQRGKEEEG
    61  VYGFLIEDIR KEVNRASKLK CCVCKKNGAS IGCVAPRCKR SYHFPCGLQR ECIFQFTGNF
   121  ASFCWDHRPV QIITSNNYRE SLPCTICLEF IEPIPSYNIL RSPCCKNAWF HRDCLQVQAI
   181  NAGVFFFRCT ICNNSDIFQK EMLRMGIHIP EKDASWELEE NAYQELLQHY ERCDVRRCRC
   241  KEGRDYNAPD SKWEIKRCQC CGSSGTHLAC SSLRSWEQNW ECLECRGIIY NSGEFQKAKK
   301  HVLPNSNNVG ITDCLLEESS PKLPRQSPGS QSKDLLRQGS KFRRNVSTLL IELGFQIKKK
   361  TKRLYINKAN IWNSALDAFR NRNFNPSYAI EVAYVIENDN FGSEHPGSKQ EFLSLLMQHL
   421  ENSSLFEGSL SKNLSLNSQA LKENLYYEAG KMLAISLVHG GPSPGFFSKT LFNCLVYGPE
   481  NTQPILDDVS DFDVAQIIIR INTATTVADL KSIINECYNY LELIGCLRLI TTLSDKYMLV
   541  KDILGYHVIQ RVHTPFESFK QGLKTLGVLE KIQAYPEAFC SILCHKPESL SAKILSELFT
   601  VHTLPDVKAL GFWNSYLQAV EDGKSTTTME DILIFATGCS SIPPAGFKPT PSIECLHVDF
   661  PVGNKCNNCL AIPITNTYKE FQENMDFTIR NTLRLEKEES SHYIGH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against G2E3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 40 nTPM

Expression across tissuesHPA

Tissue

testis: 40 nTPM
thymus: 13 nTPM
retina: 13 nTPM
bone marrow: 9.4 nTPM
lymph node: 8.5 nTPM
tonsil: 8.4 nTPM

Single-cell type

early spermatids: 1,182 nCPM
late spermatids: 357 nCPM
late primary spermatocytes: 153 nCPM
monocyte progenitors: 112 nCPM
early primary spermatocytes: 98 nCPM
erythrocyte progenitors: 97 nCPM

Immune cell

basophil: 20 nTPM
eosinophil: 13 nTPM
non-classical monocyte: 11 nTPM
MAIT T-cell: 10 nTPM
naive B-cell: 10 nTPM
memory B-cell: 9.3 nTPM

Brain region

cerebellum: 60 nTPM
white matter: 53 nTPM
basal ganglia: 50 nTPM
thalamus: 49 nTPM
hypothalamus: 46 nTPM
cerebral cortex: 46 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.32
gnomAD pLI: 0.97
gnomAD missense Z: 2.13
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads G2E3 as an antibody target. Whether an autoantibody or antibody against G2E3 could matter depends on whether native G2E3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

G2E3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label G2E3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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