FXYD1

Protein identityUniProt · HPA

UniProt accession

O00168

Gene

FXYD1

Ensembl

ENSG00000266964

Chromosome

19

Canonical length

92 aa

Protein class

Predicted membrane proteins, Transporters

Subcellular location

Nucleoplasm,Nucleoli,Cytosol

Quaternary structure

Homotetramer

OverviewNCBI Gene

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

92 residues, UniProt reviewed canonical sequence.

>O00168|FXYD1
     1  MASLGHILVF CVGLLTMAKA ESPKEHDPFT YDYQSLQIGG LVIAGILFIL GILIVLSRRC
    61  RCKFNQQQRT GEPDEEEGTF RSSIRRLSTR RR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FXYD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.52

Highest tissue expression

1,675 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.85

gnomAD pLI

0.24

gnomAD missense Z

0.53

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• chloride transport
• muscle contraction
• positive regulation of sodium ion export across plasma membrane
• potassium ion transport
• regulation of cardiac muscle cell membrane potential
• regulation of heart contraction
• sodium ion transport
• negative regulation of protein glutathionylation

Molecular functions

• chloride channel activity
• sodium channel regulator activity
• transmembrane transporter binding

Cellular components

• apical plasma membrane
• caveola
• intercalated disc
• plasma membrane
• sarcolemma
• sodium:potassium-exchanging ATPase complex
• T-tubule

Protein domainsUniProt · Pfam · InterPro

• Ion-transport regulator, FXYD family
• Ion-transport regulator, FXYD motif
• ATP1G1/PLM/MAT8 family
• Phospholemman

KeywordsUniProt

• Cell membrane
• Glutathionylation
• Ion transport
• Lipoprotein
• Membrane
• Palmitate
• Phosphoprotein
• Potassium
• Potassium transport
• Signal
• Sodium
• Sodium transport
• Sodium/potassium transport
• Transmembrane
• Transmembrane helix
• Transport

InteractionsUniProt · HPA

Protein binding partners of FXYD1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ATP1A3