Seroatlas · Human Serome Atlas

FOXN4

Forkhead box protein N4

Also known as: FOXN4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96NZ1
Gene
FOXN4
Ensembl
ENSG00000139445
Chromosome
12
Canonical length
517 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm,Mitochondria,Cytosol

OverviewNCBI Gene

Members of the winged-helix/forkhead family of transcription factors, such as FOXN4, are characterized by a 110-amino acid DNA-binding domain that can fold into a variant of the helix-turn-helix motif consisting of 3 alpha helices flanked by 2 large loops or wings. These transcription factors are involved in a variety of biologic processes as key regulators in development and metabolism (Li et al., 2004 [PubMed 15363391]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

517 residues, UniProt reviewed canonical sequence.

>Q96NZ1|FOXN4
     1  MIESDTSSIM SGIIRNSGQN HHPSPQEYRL LATTSDDDLP GDLQSLSWLT AVDVPRLQQM
    61  ASGRVDLGGP CVPHPHPGAL AGVADLHVGA TPSPLLHGPA GMAPRGMPGL GPITGHRDSM
   121  SQFPVGGQPS SGLQDPPHLY SPATQPQFPL PPGAQQCPPV GLYGPPFGVR PPYPQPHVAV
   181  HSSQELHPKH YPKPIYSYSC LIAMALKNSK TGSLPVSEIY SFMKEHFPYF KTAPDGWKNS
   241  VRHNLSLNKC FEKVENKMSG SSRKGCLWAL NLARIDKMEE EMHKWKRKDL AAIHRSMANP
   301  EELDKLISDR PESCRRPGKP GEPEAPVLTH ATTVAVAHGC LAVSQLPPQP LMTLSLQSVP
   361  LHHQVQPQAH LAPDSPAPAQ TPPLHALPDL SPSPLPHPAM GRAPVDFINI STDMNTEVDA
   421  LDPSIMDFAL QGNLWEEMKD EGFSLDTLGA FADSPLGCDL GASGLTPASG GSDQSFPDLQ
   481  VTGLYTAYST PDSVAASGTS SSSQYLGAQG NKPIALL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FOXN4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.63
Highest tissue expression
11 nTPM

Expression across tissuesHPA

Tissue

  • testis: 11 nTPM
  • cervix: 1 nTPM
  • liver: 1 nTPM
  • choroid plexus: 0.5 nTPM
  • retina: 0.4 nTPM
  • vagina: 0.4 nTPM

Single-cell type

  • respiratory deuterosomal cells: 55 nCPM
  • adipocytes: 28 nCPM
  • differentiating spermatogonia: 11 nCPM
  • late primary spermatocytes: 7.2 nCPM
  • late spermatids: 6 nCPM
  • podocytes: 5.2 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • choroid plexus: 1 nTPM
  • midbrain: 0.5 nTPM
  • amygdala: 0.4 nTPM
  • cerebellum: 0.4 nTPM
  • hypothalamus: 0.4 nTPM
  • medulla oblongata: 0.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.4
gnomAD pLI
0.86
gnomAD missense Z
1.23
DepMap mean gene effect
-0.11
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FOXN4 as an antibody target. Whether an autoantibody or antibody against FOXN4 could matter depends on whether native FOXN4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FOXN4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FOXN4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/FOXN4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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