Seroatlas · Human Serome Atlas

FMO4

Dimethylaniline monooxygenase [N-oxide-forming] 4

Also known as: FMO2, FMO4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P31512
Gene: FMO4
Ensembl: ENSG00000076258
Chromosome: 1
Canonical length: 558 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location: Vesicles,Cytosol

OverviewNCBI Gene

Metabolic N-oxidation of diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man. This results in a small subpopulation with reduced TMA N-oxidation capacity and causes fish odor syndrome (Trimethylaminuria). Three forms of the enzyme are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. [provided by RefSeq, Jan 2015]

Canonical amino-acid sequenceUniProt

558 residues, UniProt reviewed canonical sequence.

>P31512|FMO4
     1  MAKKVAVIGA GVSGLSSIKC CVDEDLEPTC FERSDDIGGL WKFTESSKDG MTRVYKSLVT
    61  NVCKEMSCYS DFPFHEDYPN FMNHEKFWDY LQEFAEHFDL LKYIQFKTTV CSITKRPDFS
   121  ETGQWDVVTE TEGKQNRAVF DAVMVCTGHF LNPHLPLEAF PGIHKFKGQI LHSQEYKIPE
   181  GFQGKRVLVI GLGNTGGDIA VELSRTAAQV LLSTRTGTWV LGRSSDWGYP YNMMVTRRCC
   241  SFIAQVLPSR FLNWIQERKL NKRFNHEDYG LSITKGKKAK FIVNDELPNC ILCGAITMKT
   301  SVIEFTETSA VFEDGTVEEN IDVVIFTTGY TFSFPFFEEP LKSLCTKKIF LYKQVFPLNL
   361  ERATLAIIGL IGLKGSILSG TELQARWVTR VFKGLCKIPP SQKLMMEATE KEQLIKRGVF
   421  KDTSKDKFDY IAYMDDIAAC IGTKPSIPLL FLKDPRLAWE VFFGPCTPYQ YRLMGPGKWD
   481  GARNAILTQW DRTLKPLKTR IVPDSSKPAS MSHYLKAWGA PVLLASLLLI CKSSLFLKLV
   541  RDKLQDRMSP YLVSLWRG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FMO4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 57 nTPM

Expression across tissuesHPA

Tissue

liver: 57 nTPM
kidney: 45 nTPM
small intestine: 8.6 nTPM
blood vessel: 7.1 nTPM
epididymis: 5.9 nTPM
duodenum: 5.1 nTPM

Single-cell type

proximal tubule cells: 121 nCPM
distal convoluted tubule cells: 94 nCPM
renal connecting tubule cells: 74 nCPM
enterocytes: 69 nCPM
hepatocytes: 52 nCPM
fibro-adipogenic progenitors: 38 nCPM

Immune cell

T-reg: 2.1 nTPM
naive B-cell: 1.8 nTPM
NK-cell: 1.7 nTPM
intermediate monocyte: 1.6 nTPM
memory CD8 T-cell: 1.3 nTPM
memory B-cell: 1.2 nTPM

Brain region

white matter: 4.6 nTPM
medulla oblongata: 4.5 nTPM
choroid plexus: 3.9 nTPM
thalamus: 3.9 nTPM
basal ganglia: 3.7 nTPM
pons: 3.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.18
gnomAD pLI: 0
gnomAD missense Z: 0.44
DepMap mean gene effect: 0.1
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

endoplasmic reticulum membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FMO4 as an antibody target. Whether an autoantibody or antibody against FMO4 could matter depends on whether native FMO4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FMO4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FMO4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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