Seroatlas · Human Serome Atlas

FHL5

Four and a half LIM domains protein 5

Also known as: ACT, dJ393D12.2, FHL5_HUMAN, FLJ33049

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q5TD97
Gene
FHL5
Ensembl
ENSG00000112214
Chromosome
6
Canonical length
284 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Polymorphisms in this gene may be associated with susceptibility for migraine headaches. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Apr 2016]

Canonical amino-acid sequenceUniProt

284 residues, UniProt reviewed canonical sequence.

>Q5TD97|FHL5
     1  MTTAHFYCQY CTASLLGKKY VLKDDSPYCV TCYDRVFSNY CEECKKPIES DSKDLCYKDR
    61  HWHEGCFKCT KCNHSLVEKP FAAKDERLLC TECYSNECSS KCFHCKRTIM PGSRKMEFKG
   121  NYWHETCFVC ENCRQPIGTK PLISKESGNY CVPCFEKEFA HYCNFCKKVI TSGGITFCDQ
   181  LWHKECFLCS GCRKDLCEEQ FMSRDDYPFC VDCYNHLYAN KCVACSKPIS GLTGAKFICF
   241  QDSQWHSECF NCGKCSVSLV GKGFLTQNKE IFCQKCGSGM DTDI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FHL5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
245 nTPM

Expression across tissuesHPA

Tissue

  • blood vessel: 245 nTPM
  • testis: 51 nTPM
  • adipose tissue: 32 nTPM
  • cervix: 23 nTPM
  • epididymis: 16 nTPM
  • breast: 15 nTPM

Single-cell type

  • late spermatids: 1,947 nCPM
  • early spermatids: 1,427 nCPM
  • vascular smooth muscle cells: 415 nCPM
  • late primary spermatocytes: 379 nCPM
  • pericytes: 241 nCPM
  • leydig cells: 134 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • basal ganglia: 11 nTPM
  • pons: 8.5 nTPM
  • thalamus: 8.1 nTPM
  • medulla oblongata: 6.8 nTPM
  • choroid plexus: 6.5 nTPM
  • cerebellum: 5.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.61
gnomAD pLI
0
gnomAD missense Z
-0.31
DepMap mean gene effect
0.01
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of FHL5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FHL5 as an antibody target. Whether an autoantibody or antibody against FHL5 could matter depends on whether native FHL5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FHL5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FHL5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/FHL5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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