FDX1

Protein identityUniProt · HPA

UniProt accession

P10109

Gene

FDX1

Ensembl

ENSG00000137714

Chromosome

11

Canonical length

184 aa

Protein class

Predicted intracellular proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

184 residues, UniProt reviewed canonical sequence.

>P10109|FDX1
     1  MAAAGGARLL RAASAVLGGP AGRWLHHAGS RAGSSGLLRN RGPGGSAEAS RSLSVSARAR
    61  SSSEDKITVH FINRDGETLT TKGKVGDSLL DVVVENNLDI DGFGACEGTL ACSTCHLIFE
   121  DHIYEKLDAI TDEENDMLDL AYGLTDRSRL GCQICLTKSM DNMTVRVPET VADARQSIDV
   181  GKTS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FDX1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.46

Highest tissue expression

300 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for FDX1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.36

gnomAD pLI

0.09

gnomAD missense Z

0.45

DepMap mean gene effect

-0.27

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to cAMP
• cellular response to forskolin
• cholesterol metabolic process
• electron transport chain
• hormone biosynthetic process
• P450-containing electron transport chain
• steroid biosynthetic process

Molecular functions

• 2 iron, 2 sulfur cluster binding
• electron transfer activity
• iron ion binding

Cellular components

• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• 2Fe-2S ferredoxin-type iron-sulfur binding domain
• Adrenodoxin-like
• Beta-grasp domain superfamily
• Adrenodoxin, iron-sulphur binding site
• 2Fe-2S ferredoxin-like superfamily
• 2Fe-2S iron-sulfur cluster binding domain

KeywordsUniProt

• 2Fe-2S
• Acetylation
• Cholesterol metabolism
• Electron transport
• Iron
• Iron-sulfur
• Lipid metabolism
• Metal-binding
• Mitochondrion
• Phosphoprotein
• Steroid metabolism
• Steroidogenesis
• Sterol metabolism
• Transit peptide
• Transport

InteractionsUniProt · HPA

Protein binding partners of FDX1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CYP11A1
• FDXR