FBXO8

Protein identityUniProt · HPA

UniProt accession

Q9NRD0

Gene

FBXO8

Ensembl

ENSG00000164117

Chromosome

4

Canonical length

319 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

319 residues, UniProt reviewed canonical sequence.

>Q9NRD0|FBXO8
     1  MGQGLWRVVR NQQLQQEGYS EQGYLTREQS RRMAASNISN TNHRKQVQGG IDIYHLLKAR
    61  KSKEQEGFIN LEMLPPELSF TILSYLNATD LCLASCVWQD LANDELLWQG LCKSTWGHCS
   121  IYNKNPPLGF SFRKLYMQLD EGSLTFNANP DEGVNYFMSK GILDDSPKEI AKFIFCTRTL
   181  NWKKLRIYLD ERRDVLDDLV TLHNFRNQFL PNALREFFRH IHAPEERGEY LETLITKFSH
   241  RFCACNPDLM RELGLSPDAV YVLCYSLILL SIDLTSPHVK NKMSKREFIR NTRRAAQNIS
   301  EDFVGHLYDN IYLIGHVAA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBXO8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Unknown

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

44 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.53

gnomAD pLI

0.49

gnomAD missense Z

1.94

DepMap mean gene effect

0.14

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of ARF protein signal transduction
• ubiquitin-dependent protein catabolic process

Molecular functions

• guanyl-nucleotide exchange factor activity

Cellular components

• ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• Sec7 domain
• F-box domain
• Sec7, C-terminal domain superfamily
• Sec7 domain superfamily
• F-box-like domain superfamily
• Sec7 domain
• F-box-like
• F-box only protein 8, F-box domain

KeywordsUniProt

• Guanine-nucleotide releasing factor

InteractionsUniProt · HPA

Protein binding partners of FBXO8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GSTP1