FBXO3

Protein identityUniProt · HPA

UniProt accession

Q9UK99

Gene

FBXO3

Ensembl

ENSG00000110429

Chromosome

11

Canonical length

471 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

471 residues, UniProt reviewed canonical sequence.

>Q9UK99|FBXO3
     1  MAAMETETAP LTLESLPTDP LLLILSFLDY RDLINCCYVS RRLSQLSSHD PLWRRHCKKY
    61  WLISEEEKTQ KNQCWKSLFI DTYSDVGRYI DHYAAIKKAW DDLKKYLEPR CPRMVLSLKE
   121  GAREEDLDAV EAQIGCKLPD DYRCSYRIHN GQKLVVPGLL GSMALSNHYR SEDLLDVDTA
   181  AGGFQQRQGL KYCLPLTFCI HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI
   241  IGATFTDWFT SYVKNVVSGG FPIIRDQIFR YVHDPECVAT TGDITVSVST SFLPELSSVH
   301  PPHYFFTYRI RIEMSKDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG EFPIISPGRV
   361  YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK IFNVAIPRFH MACPTFRVSI ARLEMGPDEY
   421  EEMEEEEEEE EEEDEDDDSA DMDESDEDDE EERRRRVFDV PIRRRRCSRL F

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBXO3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

116 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.29

gnomAD pLI

0.99

gnomAD missense Z

2.15

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• protein ubiquitination
• proteolysis
• response to lipopolysaccharide
• SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Molecular functions

• ubiquitin-like ligase-substrate adaptor activity
• ubiquitin-protein transferase activity

Cellular components

• cytoplasm
• cytosol
• nucleoplasm
• nucleus
• SCF ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• F-box domain
• ApaG domain
• Knr4/Smi1-like domain
• F-box-like domain superfamily
• ApaG domain superfamily
• Knr4/Smi1-like domain superfamily
• F-box Protein Family Substrate Recognition Components
• ApaG domain
• F-box-like
• SMI1 / KNR4 family (SUKH-1)

KeywordsUniProt

• Host-virus interaction
• Nucleus
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of FBXO3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PML
• CUL1
• SKP1