Seroatlas · Human Serome Atlas

FBXO25

F-box only protein 25

Also known as: FBX25, FBX25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8TCJ0
Gene: FBXO25
Ensembl: ENSG00000147364
Chromosome: 8
Canonical length: 367 aa
Protein class: Disease related genes, Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

367 residues, UniProt reviewed canonical sequence.

>Q8TCJ0|FBXO25
     1  MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE DGEIFNNEEH
    61  EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY CTLGEAFNRL DFSSAIQDIR
   121  RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL
   181  CILIRGVGKS VLVGNINIWI CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL
   241  YRFSDGWDII TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY
   301  FALQKHYPAK EQYGDTLHFC RHCSILFWKD YHLALLFKDS GHPCTAADPD SCFTPVSPQH
   361  FIDLFKF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBXO25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.38
Highest tissue expression: 92 nTPM

Expression across tissuesHPA

Tissue

testis: 92 nTPM
liver: 29 nTPM
pancreas: 27 nTPM
prostate: 24 nTPM
skeletal muscle: 20 nTPM
adrenal gland: 19 nTPM

Single-cell type

late primary spermatocytes: 1,195 nCPM
early spermatids: 536 nCPM
late spermatids: 375 nCPM
early primary spermatocytes: 318 nCPM
oocytes: 193 nCPM
epicardial cells: 152 nCPM

Immune cell

memory B-cell: 17 nTPM
T-reg: 13 nTPM
naive B-cell: 12 nTPM
MAIT T-cell: 12 nTPM
intermediate monocyte: 11 nTPM
memory CD4 T-cell: 11 nTPM

Brain region

cerebellum: 27 nTPM
white matter: 23 nTPM
midbrain: 22 nTPM
basal ganglia: 19 nTPM
hypothalamus: 19 nTPM
cerebral cortex: 19 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.74
gnomAD pLI: 0
gnomAD missense Z: -0.57
DepMap mean gene effect: 0
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein ubiquitination

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of FBXO25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FBXO25 as an antibody target. Whether an autoantibody or antibody against FBXO25 could matter depends on whether native FBXO25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FBXO25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FBXO25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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