FBXL8

Protein identityUniProt · HPA

UniProt accession

Q96CD0

Gene

FBXL8

Ensembl

ENSG00000135722

Chromosome

16

Canonical length

374 aa

Protein class

Predicted intracellular proteins

Subcellular location

Golgi apparatus

OverviewNCBI Gene

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. It shares 78% sequence identity with the mouse protein. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

374 residues, UniProt reviewed canonical sequence.

>Q96CD0|FBXL8
     1  MAEPGEGLPE EVLALIFRHL SLRDRAAAAR VCRAWAAAAT CSAVWHDTKI SCECELEGML
    61  PPYLSACLDH IHNLRLEFEP SRKPSRRAAI ELLMVLAGRA PGLRGLRLEC RGEKPLFDAG
   121  RDVLEAVHAV CGAASQLRHL DLRRLSFTLD DALVLQAARS CPELHSLFLD NSTLVGSVGP
   181  GSVLELLEAC PRLRALGLHL ASLSHAILEA LAAPDRAPFA LLALRCACPE DARASPLPNE
   241  AWVALRRRHP GLAVELELEP ALPAESVTRV LQPAVPVAAL RLNLSGDTVG PVRFAAHHYA
   301  ATLCALEVRA AASAELNAAL EELAARCAAL REVHCFCVVS HSVLDAFRAH CPRLRTYTLK
   361  LTREPHPWRP TLVA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBXL8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

51 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.91

gnomAD pLI

0

gnomAD missense Z

0.2

DepMap mean gene effect

-0.07

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

• cytosol

Protein domainsUniProt · Pfam · InterPro

• F-box domain
• Leucine-rich repeat domain superfamily
• F-box-like domain superfamily
• F-box-like

KeywordsUniProt

• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of FBXL8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CUL1
• SKP1