FBXL5

Protein identityUniProt · HPA

UniProt accession

Q9UKA1

Gene

FBXL5

Ensembl

ENSG00000118564

Chromosome

4

Canonical length

691 aa

Protein class

Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2010]

Canonical amino-acid sequenceUniProt

691 residues, UniProt reviewed canonical sequence.

>Q9UKA1|FBXL5
     1  MAPFPEEVDV FTAPHWRMKQ LVGLYCDKLS KTNFSNNNDF RALLQSLYAT FKEFKMHEQI
    61  ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK NEYEQLNYAK QLKERLEAFT
   121  RDFLPHMKEE EEVFQPMLME YFTYEELKDI KKKVIAQHCS QKDTAELLRG LSLWNHAEER
   181  QKFFKYSVDE KSDKEAEVSE HSTGITHLPP EVMLSIFSYL NPQELCRCSQ VSMKWSQLTK
   241  TGSLWKHLYP VHWARGDWYS GPATELDTEP DDEWVKNRKD ESRAFHEWDE DADIDESEES
   301  AEESIAISIA QMEKRLLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ ILELCPNLEH
   361  LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDVA LEKISRALGI LTSHQSGFLK
   421  TSTSKITSTA WKNKDITMQS TKQYACLHDL TNKGIGEEID NEHPWTKPVS SENFTSPYVW
   481  MLDAEDLADI EDTVEWRHRN VESLCVMETA SNFSCSTSGC FSKDIVGLRT SVCWQQHCAS
   541  PAFAYCGHSF CCTGTALRTM SSLPESSAMC RKAARTRLPR GKDLIYFGSE KSDQETGRVL
   601  LFLSLSGCYQ ITDHGLRVLT LGGGLPYLEH LNLSGCLTIT GAGLQDLVSA CPSLNDEYFY
   661  YCDNINGPHA DTASGCQNLQ CGFRACCRSG E

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBXL5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

119 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.24

gnomAD pLI

1

gnomAD missense Z

1.85

DepMap mean gene effect

0.03

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intracellular iron ion homeostasis
• multicellular organismal-level iron ion homeostasis
• positive regulation of protein catabolic process
• protein ubiquitination
• SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Molecular functions

• 2 iron, 2 sulfur cluster binding
• iron ion binding
• ubiquitin-protein transferase activity

Cellular components

• cytosol
• nucleus
• perinuclear region of cytoplasm
• SCF ubiquitin ligase complex
• ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• Leucine-rich repeat
• F-box domain
• Leucine-rich repeat, cysteine-containing subtype
• Leucine-rich repeat domain superfamily
• F-box-like domain superfamily
• F-box-like
• Leucine Rich repeat
• Hemerythrin-like
• FBXL5-like, hemerythrin-like domain
• Hemerythrin HHE cation binding domain

KeywordsUniProt

• 2Fe-2S
• Cytoplasm
• Iron
• Iron-sulfur
• Leucine-rich repeat
• Metal-binding
• Nucleus
• Repeat
• Ubl conjugation
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of FBXL5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DCTN1
• HERC2
• SKP1