EYA3

Protein identityUniProt · HPA

UniProt accession

Q99504

Gene

EYA3

Ensembl

ENSG00000158161

Chromosome

1

Canonical length

573 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Canonical amino-acid sequenceUniProt

573 residues, UniProt reviewed canonical sequence.

>Q99504|EYA3
     1  MEEEQDLPEQ PVKKAKMQES GEQTISQVSN PDVSDQKPET SSLASNLPMS EEIMTCTDYI
    61  PRSSNDYTSQ MYSAKPYAHI LSVPVSETAY PGQTQYQTLQ QTQPYAVYPQ ATQTYGLPPF
   121  GALWPGMKPE SGLIQTPSPS QHSVLTCTTG LTTSQPSPAH YSYPIQASST NASLISTSST
   181  IANIPAAAVA SISNQDYPTY TILGQNQYQA CYPSSSFGVT GQTNSDAEST TLAATTYQSE
   241  KPSVMAPAPA AQRLSSGDPS TSPSLSQTTP SKDTDDQSRK NMTSKNRGKR KADATSSQDS
   301  ELERVFLWDL DETIIIFHSL LTGSYAQKYG KDPTVVIGSG LTMEEMIFEV ADTHLFFNDL
   361  EECDQVHVED VASDDNGQDL SNYSFSTDGF SGSGGSGSHG SSVGVQGGVD WMRKLAFRYR
   421  KVREIYDKHK SNVGGLLSPQ RKEALQRLRA EIEVLTDSWL GTALKSLLLI QSRKNCVNVL
   481  ITTTQLVPAL AKVLLYGLGE IFPIENIYSA TKIGKESCFE RIVSRFGKKV TYVVIGDGRD
   541  EEIAAKQHNM PFWRITNHGD LVSLHQALEL DFL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EYA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.51

Highest tissue expression

14 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.26

gnomAD pLI

1

gnomAD missense Z

1.94

DepMap mean gene effect

-0.16

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• anatomical structure morphogenesis
• cell differentiation
• double-strand break repair
• negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
• positive regulation of DNA repair
• response to ionizing radiation
• visual perception

Molecular functions

• histone H2AXY142 phosphatase activity
• metal ion binding
• protein tyrosine phosphatase activity

Cellular components

• centrosome
• cytoplasm
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• EYA domain
• Protein phosphatase EYA
• EYA domain superfamily
• EYA domain, metazoan
• haloacid dehalogenase-like hydrolase

KeywordsUniProt

• Acetylation
• Activator
• Chromatin regulator
• Cytoplasm
• Developmental protein
• DNA damage
• DNA repair
• Hydrolase
• Magnesium
• Metal-binding
• Nucleus
• Phosphoprotein
• Protein phosphatase
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of EYA3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DACH1
• SIX1
• SIX4
• SIX5
• SIX2