EPPK1

Protein identityUniProt · HPA

UniProt accession

P58107

Gene

EPPK1

Ensembl

ENSG00000261150

Chromosome

8

Canonical length

5088 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Intermediate filaments

OverviewNCBI Gene

The protein encoded by this gene belongs to the plakin family of proteins, which play a role in the organization of cytoskeletal architecture. This family member is composed of several highly homologous plakin repeats. It may function to maintain the integrity of keratin intermediate filament networks in epithelial cells. Studies of the orthologous mouse protein suggest that it accelerates keratinocyte migration during wound healing. [provided by RefSeq, Oct 2013]

Canonical amino-acid sequenceUniProt

5088 residues, UniProt reviewed canonical sequence.

>P58107|EPPK1
     1  MSGHTLPPLP VPGTNSTEQA SVPRAMAATL GAGTPPRPQA RSIAGVYVEA SGQAQSVYAA
    61  MEQGLLPAGL GQALLEAQAA TGGLVDLARG QLLPVSKALQ QGLVGLELKE KLLAAERATT
   121  GYPDPYGGEK LALFQAIGKE VVDRALGQSW LEVQLATGGL VDPAQGVLVA PEPACHQGLL
   181  DRETWHKLSE LEPGTGDLRF LDPNTLERLT YHQLLERCVR APGSGLALLP LKITFRSMGG
   241  AVSAAELLEV GILDEQAVQG LREGRLAAVD VSARAEVRRY LEGTGSVAGV VLLPEGHKKS
   301  FFQAATEHLL PMGTALPLLE AQAATHTLVD PITGQRLWVD EAVRAGLVSP ELHEQLLVAE
   361  QAVTGHHDPF SGSQIPLFQA MKKGLVDRPL ALRLLDAQLA TGGLVCPARR LRLPLEAALR
   421  CGCLDEDTQR QLSQAGSFSD GTHGGLRYEQ LLALCVTDPE TGLAFLPLSG GPRGGEPQGP
   481  PFIKYSTRQA LSTATATVSV GKFRGRPVSL WELLFSEAIS SEQRAMLAQQ YQEGTLSVEK
   541  LAAKLSATLE QAAATARVTF SGLRDTVTPG ELLKAEIIDQ DLYERLEHGQ ATAKDVGSLA
   601  SVQRYLQGTG CIAGLLLPGS QERLSIYEAR CKGLLRPGTA LILLEAQAAT GFIIDPKANK
   661  GHSVEEALRA AVIGPDVFAK LLSAERAVTG YTDPYTGQQI SLFQAMQKGL IVREHGIRLL
   721  EAQIATGGVI DPVHSHRVPV DVAYRRGYFD QMLNLILLDP SDDTKGFFDP NTHENLTYLQ
   781  LLERCVRDPE TGLYLLPLSS TQSPLVDSAT QQAFQNLLLS VKYGRFQGQR VSAWELINSE
   841  YFSEGRRRQL LRRYRQREVT LGQVAKLLEA ETQRQADIML PALRSRVTVH QLLEAGIIDQ
   901  QLLDQVLAGT ISPEALLLMD GVRRYLCGLG AVGGVRLLPS GQRLSLYQAM RQKLLGPRVA
   961  LALLEAQAAT GTIMDPHSPE SLSVDEAVRR GVVGPELYGR LKRAEGAIAG FRDPFSGKQV
  1021  SVFQAMKKGL IPWEQAARLL EAQVATGGII DPTSHHHLPM PVAIQRGYVD QEMETALSSS
  1081  SETFPTPDGQ GRTSYAQLLE ECPRDETSGL HLLPLPESAP ALPTEEQVQR SLQAVPGAKD
  1141  GTSLWDLLSS CHFTEEQRRG LLEDVQEGRT TVPQLLASVQ RWVQETKLLA QARVMVPGPR
  1201  GEVPAVWLLD AGIITQETLE ALAQGTQSPA QVAEQPAVKA CLWGTGCVAG VLLQPSGAKA
  1261  SIAQAVRDGL LPTGLGQRLL EAQVASGFLV DPLNNQRLSV EDAVKVGLVG RELSEQLGQA
  1321  ERAAAGYPDP YSRASLSLWQ AMEKGLVPQN EGLPLLQVQL ATGGVVDPVH GVHLPQAAAC
  1381  RLGLLDTQTS QVLTAVDKDN KFFFDPSARD QVTYQQLRER CVCDSETGLL LLPLPSDTVL
  1441  EVDDHTAVAL RAMKVPVSTG RFKGCSVSLW DLLLSEYVGA DKRRELVALC RSGRAAALRQ
  1501  VVSAVTTLVE AAERQPLQAT FRGLRKQVSA RDLFRAQLIS RKTLDELSQG TTTVKEVAEM
  1561  DSVKRSLEGG NFIAGVLIQG TQERMSIPEA LRRHILRPGT ALVLLEAQAA TGFIIDPVEN
  1621  RKLTVEEAFK AGMFGKETYV KLLSAERAVT GYTDPYTGQQ ISLFQAMQKD LIVREHGIRL
  1681  LEAQIATGGI IDPVHSHRVP VDVAYRCGYF DEEMNRILAD PSDDTKGFFD PNTHENLTYL
  1741  QLLERCVEDP ETGLYLLQII KKGENYVYIN EATRHVLQSR TAKMRVGRFA DQVVSFWDLL
  1801  SSPYFTEDRK RELIQEYGAQ SGGLEKLLEI ITTTIEETET QNQGIKVAAI RGEVTAADLF
  1861  NSRVIDQKTL HTLRVGRTGG QALSTLECVK PYLEGSGCIA GVTVPSTREV MSLHEASRKE
  1921  LIPAAFATWL LEAQAATGFL LDPCTRQKLS VDEAVDVGLV NEELRERLLK AERAATGYRD
  1981  PATGDTIPLF QAMQKQLIEK AEALRLLEVQ VATGGVIDPQ HHHRLPLETA YRRGCLHKDI
  2041  YALISDQKHM RKRFVDPNTQ EKVSYRELQE RCRPQEDTGW LLFPVNKAAR DSEHIDDETR
  2101  RALEAEQVEI TVGRFRGQKP TLWALLNSEY VTEEKKLQLV RMYRTHTRRA LQTVAQLILE
  2161  LIEKQETSNK HLWFQGIRRQ ITASELLSSA IITEEMLQDL ETGRSTTQEL MEDDRVKRYL
  2221  EGTSCIAGVL VPAKDQPGRQ EKMSIYQAMW KGVLRPGTAL VLLEAQAATG FVIDPVRNLR
  2281  LSVEEAVAAG VVGGEIQEKL LSAERAVTGY TDPYTGQQIS LFQAMQKDLI VREHGIRLLE
  2341  AQIATGGVID PVHSHRVPVD VAYRRGYFDE EMNRVLADPS DDTKGFFDPN THENLTYVQL
  2401  LRRCVPDPDT GLYMLQLAGR GSAVHQLSEE LRCALRDARV TPGSGALQGQ SVSVWELLFY
  2461  REVSEDRRQD LLSRYRAGTL TVEELGATLT SLLAQAQAQA RAEAEAGSPR PDPREALRAA
  2521  TMEVKVGRLR GRAVPVWDVL ASGYVSRAAR EELLAEFGSG TLDLPALTRR LTAIIEEAEE
  2581  APGARPQLQD AWRGPREPGP AGRGDGDSGR SQREGQGEGE TQEAAAAAAA AAARRQEQTL
  2641  RDATMEVQRG QFQGRPVSVW DVLFSSYLSE ARRDELLAQH AAGALGLPDL VAVLTRVIEE
  2701  TEERLSKVSF RGLRRQVSAS ELHTSGILGP ETLRDLAQGT KTLQEVTEMD SVKRYLEGTS
  2761  CIAGVLVPAK DQPGRQEKMS IYQAMWKGVL RPGTALVLLE AQAATGFVID PVRNLRLSVE
  2821  EAVAAGVVGG EIQEKLLSAE RAVTGYTDPY TGQQISLFQA MQKDLIVREH GIRLLEAQIA
  2881  TGGVIDPVHS HRVPVDVAYQ RGYFDEEMNR VLADPSDDTK GFFDPNTHEN LTYVQLLRRC
  2941  VPDPDTGLYM LQLAGRGSAV HQLSEELRCA LRDARVTPGS GALQGQSVSV WELLFYREVS
  3001  EDRRQDLLSR YRAGTLTVEE LGATLTSLLA QAQAQARAEA EAGSPRPDPR EALRAATMEV
  3061  KVGRLRGRAV PVWDVLASGY VSRAAREELL AEFGSGTLDL PALTRRLTAI IEEAEEAPGA
  3121  RPQLQDAWRG PREPGPAGRG DGDSGRSQRE GQGEGETQEA AAAARRQEQT LRDATMEVQR
  3181  GQFQGRPVSV WDVLFSSYLS EARRDELLAQ HAAGALGLPD LVAVLTRVIE ETEERLSKVS
  3241  FRGLRCQVSA SELHTSGILG PETLRDLAQG TKTLQEVTEM DSVKRYLEGT SCIAGVLVPA
  3301  KDQPGRQEKM SIYQAMWKGV LRPGTALVLL EAQAATGFVI DPVRNLRLSV EEAVAAGVVG
  3361  GEIQEKLLSA ERAVTGYTDP YTGQQISLFQ AMQKDLIVRE HGIRLLEAQI ATGGVIDPVH
  3421  SHRVPVDVAY RRGYFDEEMN RVLADPSDDT KGFFDPNTHE NLTYVQLLRR CVPDPDTGLY
  3481  MLQLAGRGSA VHQLSEELRC ALRDARVTPG SGALQGQSVS VWELLFYREV SEDRRQDLLS
  3541  RYRAGTLTVE ELGATLTSLL AQAQAQARAE AEAGSPRPDP REALRAATME VKVGRLRGRA
  3601  VPVWDVLASG YVSRAAREEL LAEFGSGTLD LPALTRRLTA IIEEAEEAPG ARPQLQDAWR
  3661  GPREPGPAGR GDGDSGRSQR EGQGEGETQE AAAATAAARR QEQTLRDATM EVQRGQFQGR
  3721  PVSVWDVLFS SYLSEARRDE LLAQHAAGAL GLPDLVAVLT RVIEETEERL SKVSFRGLRR
  3781  QVSASELHTS GILGPETLRD LAQGTKTLQE VTEMDSVKRY LEGTSCIAGV LVPAKDQPGR
  3841  QEKMSIYQAM WKGVLRPGTA LVLLEAQAAT GFVIDPVRNL RLSVEEAVAA GVVGGEIQEK
  3901  LLSAERAVTG YTDPYTGQQI SLFQAMQKDL IVREHGIRLL EAQIATGGVI DPVHSHRVPV
  3961  DVAYRRGYFD EEMNRVLADP SDDTKGFFDP NTHENLTYVQ LLRRCVPDPD TGLYMLQLAG
  4021  RGSAVHQLSE ELRCALRDAR VTPGSGALQG QSVSVWELLF YREVSEDRRQ DLLSRYRAGT
  4081  LTVEELGATL TSLLAQAQAQ ARAEAEAGSP RPDPREALRA ATMEVKVGRL RGRAVPVWDV
  4141  LASGYVSRAA REELLAEFGS GTLDLPALTR RLTAIIEEAE EAPGARPQLQ DAWRGPREPG
  4201  PAGRGDGDSG RSQREGQGEG ETQEAAAATA AARRQEQTLR DATMEVQRGQ FQGRPVSVWD
  4261  VLFSSYLSEA RRDELLAQHA AGALGLPDLV AVLTRVIEET EERLSKVSFR GLRRQVSASE
  4321  LHTSGILGPE TLRDLAQGTK TLQEVTEMDS VKRYLEGTSC IAGVLVPAKD QPGHQEKMSI
  4381  YQAMWKGVLR PGTALVLLEA QAATGFVIDP VRNLRLSVEE AVAAGVVGGE IQEKLLSAER
  4441  AVTGYTDPYT GQQISLFQAM QKDLIVREHG IRLLEAQIAT GGVIDPVHSH RVPVDVAYRR
  4501  GYFDEEMNRV LAHPSDDTKG FFDPNTHENL TYVQLLRRCV PDPDTGLYML QLAGRGSAVH
  4561  QLSEELRCAL RDARVMPGSG ALQGQSVSVW ELLFYREVSE DRRQDLLSRY RAGTLTVEEL
  4621  GATLTSLLAQ AQAQARAEAE AEAGSPRPDP REALRAATME VKVGRLRGRA VPVWDVLASG
  4681  YVSGAAREEL LAEFGSGTLD LPALTRRLTA IIEEAEEAPG ARPQLQDAWR GPREPGPAGR
  4741  GDGDSGRSQR EGQGEGETQE AAAAARRQEQ TLRDATMEVQ RGQFQGRPVS VWDVLFSSYL
  4801  SEAHRDELLA QHAAGALGLP DLVAVLTRVI EETEERLSKV SFRGLRRQVS ASELHTSGIL
  4861  GPETLRDLAQ GTKTLQEVTE MDSVKRYLEG TSCIAGVLVP AKDQPGRQEK MSIYQAMWKG
  4921  VLRPGTALVL LEAQAATGFV IDPVRNLRLS VEEAVAAGVV GGEIQEKLLS AERAVTGYTD
  4981  PYTGQQISLF QAMQKDLIVR EHGIRLLEAQ IATGGVIDPV HSHRVPVDVA YRRGYFDEEM
  5041  NRVLADPSDD TKGFFDPNTH ENLTYLQLLQ RATLDPETGL LFLSLSLQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EPPK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0

Highest tissue expression

15 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for EPPK1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.2

gnomAD pLI

0

gnomAD missense Z

-3.04

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intermediate filament bundle assembly
• intermediate filament organization
• negative regulation of cell migration
• negative regulation of epithelial cell proliferation
• negative regulation of keratinocyte migration
• negative regulation of keratinocyte proliferation
• negative regulation of wound healing
• wound healing
• regulation of epithelium regeneration

Molecular functions

• intermediate filament binding
• keratin filament binding
• RNA binding
• structural molecule activity

Cellular components

• apicolateral plasma membrane
• basolateral plasma membrane
• bicellular tight junction
• cell junction
• cell periphery
• cell projection
• cytoplasm
• cytoskeleton
• hemidesmosome
• intermediate filament cytoskeleton
• keratin filament
• membrane
• perinucleolar compartment

Protein domainsUniProt · Pfam · InterPro

• Plectin repeat
• Plakin repeat superfamily
• Plakin
• Plectin repeat

KeywordsUniProt

• Cell junction
• Cell membrane
• Cell projection
• Coiled coil
• Cytoplasm
• Cytoskeleton
• Membrane
• Phosphoprotein
• Repeat
• Tight junction

InteractionsUniProt · HPA

Protein binding partners of EPPK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DES
• KRT1
• KRT14
• KRT18
• KRT5
• KRT8
• VIM