EPHX1

Protein identityUniProt · HPA

UniProt accession

P07099

Gene

EPHX1

Ensembl

ENSG00000143819

Chromosome

1

Canonical length

455 aa

Protein class

Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins

OverviewNCBI Gene

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008]

Canonical amino-acid sequenceUniProt

455 residues, UniProt reviewed canonical sequence.

>P07099|EPHX1
     1  MWLEILLTSV LGFAIYWFIS RDKEETLPLE DGWWGPGTRS AAREDDSIRP FKVETSDEEI
    61  HDLHQRIDKF RFTPPLEDSC FHYGFNSNYL KKVISYWRNE FDWKKQVEIL NRYPHFKTKI
   121  EGLDIHFIHV KPPQLPAGHT PKPLLMVHGW PGSFYEFYKI IPLLTDPKNH GLSDEHVFEV
   181  ICPSIPGYGF SEASSKKGFN SVATARIFYK LMLRLGFQEF YIQGGDWGSL ICTNMAQLVP
   241  SHVKGLHLNM ALVLSNFSTL TLLLGQRFGR FLGLTERDVE LLYPVKEKVF YSLMRESGYM
   301  HIQCTKPDTV GSALNDSPVG LAAYILEKFS TWTNTEFRYL EDGGLERKFS LDDLLTNVML
   361  YWTTGTIISS QRFYKENLGQ GWMTQKHERM KVYVPTGFSA FPFELLHTPE KWVRFKYPKL
   421  ISYSYMVRGG HFAAFEEPEL LAQDIRKFLS VLERQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EPHX1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

1,594 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about EPHX1.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 143 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Hereditary lipodystrophy

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.13

gnomAD pLI

0

gnomAD missense Z

-0.06

DepMap mean gene effect

0.11

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• arachidonate metabolic process
• epoxide metabolic process
• response to toxic substance
• xenobiotic metabolic process
• hydrocarbon catabolic process

Molecular functions

• epoxide hydrolase activity
• oxysterol binding
• cis-stilbene-oxide hydrolase activity

Cellular components

• endoplasmic reticulum membrane

Protein domainsUniProt · Pfam · InterPro

• Alpha/beta hydrolase fold-1
• Epoxide hydrolase-like
• Alpha/Beta hydrolase fold
• alpha/beta hydrolase fold
• Epoxide hydrolase

KeywordsUniProt

• Aromatic hydrocarbons catabolism
• Detoxification
• Endoplasmic reticulum
• Hydrolase
• Lipid metabolism
• Membrane
• Methylation
• Microsome
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of EPHX1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MFSD8