Seroatlas · Human Serome Atlas

ENPP4

Bis(5'-adenosyl)-triphosphatase ENPP4

Also known as: AP3Aase, ENPP4_HUMAN, KIAA0879, NPP4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y6X5
Gene: ENPP4
Ensembl: ENSG00000001561
Chromosome: 6
Canonical length: 453 aa
Protein class: Enzymes, Predicted membrane proteins

OverviewNCBI Gene

Enables bis(5'-adenosyl)-triphosphatase activity. Involved in positive regulation of blood coagulation and purine ribonucleoside catabolic process. Located in extracellular exosome and membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

453 residues, UniProt reviewed canonical sequence.

>Q9Y6X5|ENPP4
     1  MKLLVILLFS GLITGFRSDS SSSLPPKLLL VSFDGFRADY LKNYEFPHLQ NFIKEGVLVE
    61  HVKNVFITKT FPNHYSIVTG LYEESHGIVA NSMYDAVTKK HFSDSNDKDP FWWNEAVPIW
   121  VTNQLQENRS SAAAMWPGTD VPIHDTISSY FMNYNSSVSF EERLNNITMW LNNSNPPVTF
   181  ATLYWEEPDA SGHKYGPEDK ENMSRVLKKI DDLIGDLVQR LKMLGLWENL NVIITSDHGM
   241  TQCSQDRLIN LDSCIDHSYY TLIDLSPVAA ILPKINRTEV YNKLKNCSPH MNVYLKEDIP
   301  NRFYYQHNDR IQPIILVADE GWTIVLNESS QKLGDHGYDN SLPSMHPFLA AHGPAFHKGY
   361  KHSTINIVDI YPMMCHILGL KPHPNNGTFG HTKCLLVDQW CINLPEAIAI VIGSLLVLTM
   421  LTCLIIIMQN RLSVPRPFSR LQLQEDDDDP LIG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENPP4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 36 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 36 nTPM
spinal cord: 33 nTPM
tongue: 24 nTPM
parathyroid gland: 21 nTPM
skeletal muscle: 21 nTPM
salivary gland: 20 nTPM

Single-cell type

salivary duct cells: 154 nCPM
neutrophil progenitors: 152 nCPM
conjunctival goblet cells: 126 nCPM
oligodendrocytes: 101 nCPM
pituicytes/fscs: 77 nCPM
parietal cells: 71 nCPM

Immune cell

gdT-cell: 9.2 nTPM
memory CD8 T-cell: 6.8 nTPM
naive CD8 T-cell: 5.8 nTPM
MAIT T-cell: 5.4 nTPM
NK-cell: 4.4 nTPM
memory CD4 T-cell: 3.4 nTPM

Brain region

white matter: 61 nTPM
medulla oblongata: 43 nTPM
basal ganglia: 43 nTPM
cerebellum: 41 nTPM
midbrain: 40 nTPM
spinal cord: 39 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.66
gnomAD pLI: 0.12
gnomAD missense Z: 0.8
DepMap mean gene effect: 0.1
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

blood coagulation
positive regulation of blood coagulation
purine ribonucleoside catabolic process

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ENPP4 as an antibody target. Whether an autoantibody or antibody against ENPP4 could matter depends on whether native ENPP4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ENPP4 is annotated at the cell surface, where native ENPP4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ENPP4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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