Seroatlas · Human Serome Atlas

ENPP3

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Also known as: B10, CD203c, ENPP3_HUMAN, gp130RB13-6, PD-IBETA, PDNP3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O14638
Gene
ENPP3
Ensembl
ENSG00000154269
Chromosome
6
Canonical length
875 aa
Protein class
CD markers, Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted secreted proteins
Secretome location
Secreted - unknown location
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Oct 2015]

Canonical amino-acid sequenceUniProt

875 residues, UniProt reviewed canonical sequence.

>O14638|ENPP3
     1  MESTLTLATE QPVKKNTLKK YKIACIVLLA LLVIMSLGLG LGLGLRKLEK QGSCRKKCFD
    61  ASFRGLENCR CDVACKDRGD CCWDFEDTCV ESTRIWMCNK FRCGETRLEA SLCSCSDDCL
   121  QRKDCCADYK SVCQGETSWL EENCDTAQQS QCPEGFDLPP VILFSMDGFR AEYLYTWDTL
   181  MPNINKLKTC GIHSKYMRAM YPTKTFPNHY TIVTGLYPES HGIIDNNMYD VNLNKNFSLS
   241  SKEQNNPAWW HGQPMWLTAM YQGLKAATYF WPGSEVAING SFPSIYMPYN GSVPFEERIS
   301  TLLKWLDLPK AERPRFYTMY FEEPDSSGHA GGPVSARVIK ALQVVDHAFG MLMEGLKQRN
   361  LHNCVNIILL ADHGMDQTYC NKMEYMTDYF PRINFFYMYE GPAPRIRAHN IPHDFFSFNS
   421  EEIVRNLSCR KPDQHFKPYL TPDLPKRLHY AKNVRIDKVH LFVDQQWLAV RSKSNTNCGG
   481  GNHGYNNEFR SMEAIFLAHG PSFKEKTEVE PFENIEVYNL MCDLLRIQPA PNNGTHGSLN
   541  HLLKVPFYEP SHAEEVSKFS VCGFANPLPT ESLDCFCPHL QNSTQLEQVN QMLNLTQEEI
   601  TATVKVNLPF GRPRVLQKNV DHCLLYHREY VSGFGKAMRM PMWSSYTVPQ LGDTSPLPPT
   661  VPDCLRADVR VPPSESQKCS FYLADKNITH GFLYPPASNR TSDSQYDALI TSNLVPMYEE
   721  FRKMWDYFHS VLLIKHATER NGVNVVSGPI FDYNYDGHFD APDEITKHLA NTDVPIPTHY
   781  FVVLTSCKNK SHTPENCPGW LDVLPFIIPH RPTNVESCPE GKPEALWVEE RFTAHIARVR
   841  DVELLTGLDF YQDKVQPVSE ILQLKTYLPT FETTI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENPP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.22
Highest tissue expression
42 nTPM

Expression across tissuesHPA

Tissue

  • small intestine: 42 nTPM
  • cervix: 30 nTPM
  • salivary gland: 17 nTPM
  • duodenum: 15 nTPM
  • seminal vesicle: 15 nTPM
  • kidney: 9.4 nTPM

Single-cell type

  • endometrial luminal cells: 934 nCPM
  • mast cells: 394 nCPM
  • endometrial glandular cells: 376 nCPM
  • medullary thymic epithelial cells: 190 nCPM
  • enterocytes: 169 nCPM
  • lacrimal acinar cells: 157 nCPM

Immune cell

  • basophil: 31 nTPM
  • NK-cell: 0.3 nTPM
  • MAIT T-cell: 0.2 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • cerebellum: 1.7 nTPM
  • midbrain: 1 nTPM
  • thalamus: 0.9 nTPM
  • hypothalamus: 0.8 nTPM
  • medulla oblongata: 0.8 nTPM
  • pons: 0.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.02
gnomAD pLI
0
gnomAD missense Z
0.07
DepMap mean gene effect
-0.08
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ENPP3 as an antibody target. Whether an autoantibody or antibody against ENPP3 could matter depends on whether native ENPP3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ENPP3 is annotated at the cell surface, where native ENPP3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ENPP3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ENPP3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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