Seroatlas · Human Serome Atlas

ENOPH1

Enolase-phosphatase E1

Also known as: E1, ENOPH_HUMAN, MASA, mtnC

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UHY7
Gene: ENOPH1
Ensembl: ENSG00000145293
Chromosome: 4
Canonical length: 261 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli,Nuclear bodies

OverviewNCBI Gene

Enables acireductone synthase activity. Involved in L-methionine salvage from methylthioadenosine. Predicted to be located in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

261 residues, UniProt reviewed canonical sequence.

>Q9UHY7|ENOPH1
     1  MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR
    61  KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA
   121  AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF
   181  DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD
   241  DEKTYYSLIT SFSELYLPSS T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENOPH1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 129 nTPM

Expression across tissuesHPA

Tissue

spinal cord: 129 nTPM
midbrain: 89 nTPM
hippocampal formation: 75 nTPM
amygdala: 73 nTPM
cerebral cortex: 67 nTPM
basal ganglia: 63 nTPM

Single-cell type

cytotrophoblasts: 118 nCPM
migrating cytotrophoblasts: 107 nCPM
esophageal suprabasal cells: 81 nCPM
hofbauer cells: 81 nCPM
esophageal basal cells: 79 nCPM
parietal cells: 74 nCPM

Immune cell

T-reg: 30 nTPM
naive CD4 T-cell: 30 nTPM
MAIT T-cell: 27 nTPM
memory CD4 T-cell: 26 nTPM
memory CD8 T-cell: 25 nTPM
NK-cell: 25 nTPM

Brain region

white matter: 83 nTPM
medulla oblongata: 67 nTPM
cerebellum: 65 nTPM
basal ganglia: 64 nTPM
spinal cord: 63 nTPM
pons: 61 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.82
gnomAD pLI: 0.01
gnomAD missense Z: 1.83
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

L-methionine salvage from methylthioadenosine

Molecular functions

magnesium ion binding
acireductone synthase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ENOPH1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

RNF41

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ENOPH1 as an antibody target. Whether an autoantibody or antibody against ENOPH1 could matter depends on whether native ENOPH1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ENOPH1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ENOPH1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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