Seroatlas · Human Serome Atlas

EHD4

EH domain-containing protein 4

Also known as: EHD4_HUMAN, PAST4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H223
Gene
EHD4
Ensembl
ENSG00000103966
Chromosome
15
Canonical length
541 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Plasma membrane,Primary cilium,Primary cilium transition zone
Quaternary structure
Homooligomer

OverviewNCBI Gene

Enables cadherin binding activity. Involved in endocytic recycling and protein homooligomerization. Located in endoplasmic reticulum and recycling endosome membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

541 residues, UniProt reviewed canonical sequence.

>Q9H223|EHD4
     1  MFSWMGRQAG GRERAGGADA VQTVTGGLRS LYLRKVLPLE EAYRFHEFHS PALEDADFEN
    61  KPMILLVGQY STGKTTFIRY LLEQDFPGMR IGPEPTTDSF IAVMYGETEG STPGNALVVD
   121  PKKPFRKLSR FGNAFLNRFM CSQLPNQVLK SISVIDSPGI LSGEKQRISR GYDFCQVLQW
   181  FAERVDRIIL LFDAHKLDIS DEFSEAIKAF RGQDDKIRVV LNKADQVDTQ QLMRVYGALM
   241  WSLGKVINTP EVLRVYIGSF WAQPLQNTDN RRLFEAEAQD LFRDIQSLPQ KAAVRKLNDL
   301  IKRARLAKVH AYIISYLKKE MPSVFGKENK KRELISRLPE IYIQLQREYQ ISAGDFPEVK
   361  AMQEQLENYD FTKFHSLKPK LIEAVDNMLS NKISPLMNLI SQEETSTPTQ LVQGGAFDGT
   421  TEGPFNQGYG EGAKEGADEE EWVVAKDKPV YDELFYTLSP INGKISGVNA KKEMVTSKLP
   481  NSVLGKIWKL ADCDCDGMLD EEEFALAKHL IKIKLDGYEL PSSLPPHLVP PSHRKSLPKA
   541  D

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EHD4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
25 nTPM

Expression across tissuesHPA

Tissue

  • heart muscle: 25 nTPM
  • urinary bladder: 22 nTPM
  • adipose tissue: 21 nTPM
  • thyroid gland: 17 nTPM
  • breast: 16 nTPM
  • blood vessel: 15 nTPM

Single-cell type

  • urothelial cells: 436 nCPM
  • esophageal apical cells: 305 nCPM
  • cardiomyocytes: 203 nCPM
  • vascular endothelial cells: 201 nCPM
  • prostatic hillock cells: 183 nCPM
  • prostatic club cells: 178 nCPM

Immune cell

  • myeloid DC: 15 nTPM
  • classical monocyte: 14 nTPM
  • intermediate monocyte: 12 nTPM
  • eosinophil: 9 nTPM
  • T-reg: 7.9 nTPM
  • total PBMC: 7.9 nTPM

Brain region

  • cerebellum: 14 nTPM
  • white matter: 12 nTPM
  • choroid plexus: 9 nTPM
  • spinal cord: 8.9 nTPM
  • medulla oblongata: 8.1 nTPM
  • pons: 7 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about EHD4.

Disease | ImmuneIEDB

Conditions an epitope on EHD4 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.79
gnomAD pLI
0
gnomAD missense Z
1.55
DepMap mean gene effect
0.01
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EHD4 as an antibody target. Whether an autoantibody or antibody against EHD4 could matter depends on whether native EHD4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EHD4 is annotated at the cell surface, where native EHD4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EHD4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EHD4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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