Seroatlas · Human Serome Atlas

EFNA4

Ephrin-A4

Also known as: EFNA4_HUMAN, EPLG4, LERK4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P52798
Gene
EFNA4
Ensembl
ENSG00000243364
Chromosome
1
Canonical length
201 aa
Protein class
Predicted membrane proteins, Predicted secreted proteins, RAS pathway related proteins
Secretome location
Secreted to blood

OverviewNCBI Gene

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin that has been implicated in proliferation and metastasis of several types of cancers. [provided by RefSeq, May 2022]

Canonical amino-acid sequenceUniProt

201 residues, UniProt reviewed canonical sequence.

>P52798|EFNA4
     1  MRLLPLLRTV LWAAFLGSPL RGGSSLRHVV YWNSSNPRLL RGDAVVELGL NDYLDIVCPH
    61  YEGPGPPEGP ETFALYMVDW PGYESCQAEG PRAYKRWVCS LPFGHVQFSE KIQRFTPFSL
   121  GFEFLPGETY YYISVPTPES SGQCLRLQVS VCCKERKSES AHPVGSPGES GTSGWRGGDT
   181  PSPLCLLLLL LLLILRLLRI L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EFNA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • skin: 29 nTPM
  • esophagus: 18 nTPM
  • vagina: 14 nTPM
  • salivary gland: 13 nTPM
  • cervix: 12 nTPM
  • epididymis: 12 nTPM

Single-cell type

  • microglia: 2.2 nCPM
  • astrocytes: 1.7 nCPM
  • ependymal cells: 1.7 nCPM
  • choroid plexus epithelial cells: 1.3 nCPM
  • gastric chief cells: 1.1 nCPM
  • oligodendrocytes: 1.1 nCPM

Immune cell

  • plasmacytoid DC: 25 nTPM
  • memory B-cell: 23 nTPM
  • naive B-cell: 17 nTPM
  • non-classical monocyte: 14 nTPM
  • naive CD4 T-cell: 12 nTPM
  • neutrophil: 11 nTPM

Brain region

  • choroid plexus: 4.4 nTPM
  • medulla oblongata: 2.6 nTPM
  • white matter: 2.5 nTPM
  • pons: 2.4 nTPM
  • midbrain: 2.3 nTPM
  • spinal cord: 2.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.46
gnomAD pLI
0
gnomAD missense Z
0.76
DepMap mean gene effect
-0.08
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EFNA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EFNA4 as an antibody target. Whether an autoantibody or antibody against EFNA4 could matter depends on whether native EFNA4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EFNA4 is annotated at the cell surface, where native EFNA4 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EFNA4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EFNA4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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