Seroatlas · Human Serome Atlas

EFNA1

Ephrin-A1

Also known as: ECKLG, EFNA1_HUMAN, EPLG1, GMAN, LERK1, TNFAIP4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P20827
Gene
EFNA1
Ensembl
ENSG00000169242
Chromosome
1
Canonical length
205 aa
Protein class
Cancer-related genes, Predicted secreted proteins, RAS pathway related proteins
Secretome location
Secreted to blood
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

205 residues, UniProt reviewed canonical sequence.

>P20827|EFNA1
     1  MEFLWAPLLG LCCSLAAADR HTVFWNSSNP KFRNEDYTIH VQLNDYVDII CPHYEDHSVA
    61  DAAMEQYILY LVEHEEYQLC QPQSKDQVRW QCNRPSAKHG PEKLSEKFQR FTPFTLGKEF
   121  KEGHSYYYIS KPIHQHEDRC LRLKVTVSGK ITHSPQAHDN PQEKRLAADD PEVRVLHSIG
   181  HSAAPRLFPL AWTVLLLPLL LLQTP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EFNA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.43
Highest tissue expression
477 nTPM

Expression across tissuesHPA

Tissue

  • liver: 477 nTPM
  • skin: 132 nTPM
  • placenta: 103 nTPM
  • lung: 92 nTPM
  • thyroid gland: 86 nTPM
  • prostate: 85 nTPM

Single-cell type

  • extravillous trophoblasts: 1,373 nCPM
  • migrating cytotrophoblasts: 583 nCPM
  • syncytiotrophoblasts: 299 nCPM
  • alveolar cells type 1: 299 nCPM
  • prostatic club cells: 256 nCPM
  • breast secretory cells: 252 nCPM

Immune cell

  • naive CD4 T-cell: 12 nTPM
  • naive CD8 T-cell: 5.9 nTPM
  • total PBMC: 1.6 nTPM
  • memory CD4 T-cell: 1 nTPM
  • gdT-cell: 0.8 nTPM
  • memory CD8 T-cell: 0.7 nTPM

Brain region

  • white matter: 32 nTPM
  • medulla oblongata: 25 nTPM
  • basal ganglia: 21 nTPM
  • midbrain: 20 nTPM
  • cerebral cortex: 20 nTPM
  • pons: 19 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.63
gnomAD pLI
0.46
gnomAD missense Z
0.83
DepMap mean gene effect
0.01
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EFNA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EFNA1 as an antibody target. Whether an autoantibody or antibody against EFNA1 could matter depends on whether native EFNA1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EFNA1 is annotated at the cell surface, where native EFNA1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EFNA1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EFNA1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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