Seroatlas · Human Serome Atlas

EEF2K

Eukaryotic elongation factor 2 kinase

Also known as: CaMKIII, eEF-2K, EF2K_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O00418
Gene
EEF2K
Ensembl
ENSG00000103319
Chromosome
16
Canonical length
725 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoplasm
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

725 residues, UniProt reviewed canonical sequence.

>O00418|EEF2K
     1  MADEDLIFRL EGVDGGQSPR AGHDGDSDGD SDDEEGYFIC PITDDPSSNQ NVNSKVNKYY
    61  SNLTKSERYS SSGSPANSFH FKEAWKHAIQ KAKHMPDPWA EFHLEDIATE RATRHRYNAV
   121  TGEWLDDEVL IKMASQPFGR GAMRECFRTK KLSNFLHAQQ WKGASNYVAK RYIEPVDRDV
   181  YFEDVRLQME AKLWGEEYNR HKPPKQVDIM QMCIIELKDR PGKPLFHLEH YIEGKYIKYN
   241  SNSGFVRDDN IRLTPQAFSH FTFERSGHQL IVVDIQGVGD LYTDPQIHTE TGTDFGDGNL
   301  GVRGMALFFY SHACNRICES MGLAPFDLSP RERDAVNQNT KLLQSAKTIL RGTEEKCGSP
   361  QVRTLSGSRP PLLRPLSENS GDENMSDVTF DSLPSSPSSA TPHSQKLDHL HWPVFSDLDN
   421  MASRDHDHLD NHRESENSGD SGYPSEKRGE LDDPEPREHG HSYSNRKYES DEDSLGSSGR
   481  VCVEKWNLLN SSRLHLPRAS AVALEVQRLN ALDLEKKIGK SILGKVHLAM VRYHEGGRFC
   541  EKGEEWDQES AVFHLEHAAN LGELEAIVGL GLMYSQLPHH ILADVSLKET EENKTKGFDY
   601  LLKAAEAGDR QSMILVARAF DSGQNLSPDR CQDWLEALHW YNTALEMTDC DEGGEYDGMQ
   661  DEPRYMMLAR EAEMLFTGGY GLEKDPQRSG DLYTQAAEAA MEAMKGRLAN QYYQKAEEAW
   721  AQMEE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EEF2K can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
46 nTPM

Expression across tissuesHPA

Tissue

  • skin: 46 nTPM
  • cerebellum: 25 nTPM
  • skeletal muscle: 24 nTPM
  • urinary bladder: 19 nTPM
  • adipose tissue: 19 nTPM
  • colon: 19 nTPM

Single-cell type

  • late spermatids: 96 nCPM
  • astrocytes: 60 nCPM
  • oligodendrocyte progenitor cells: 58 nCPM
  • choroid plexus epithelial cells: 57 nCPM
  • brain excitatory neurons: 51 nCPM
  • renal collecting duct principal cells: 50 nCPM

Immune cell

  • plasmacytoid DC: 0.4 nTPM
  • neutrophil: 0.3 nTPM
  • basophil: 0.2 nTPM
  • classical monocyte: 0.1 nTPM
  • eosinophil: 0.1 nTPM
  • MAIT T-cell: 0.1 nTPM

Brain region

  • cerebellum: 2.8 nTPM
  • thalamus: 2.5 nTPM
  • hypothalamus: 2.2 nTPM
  • pons: 2.2 nTPM
  • medulla oblongata: 2 nTPM
  • white matter: 2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.08
gnomAD pLI
0
gnomAD missense Z
0.61
DepMap mean gene effect
-0.03
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EEF2K as an antibody target. Whether an autoantibody or antibody against EEF2K could matter depends on whether native EEF2K is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EEF2K is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EEF2K as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EEF2K. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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