DUSP4

Protein identityUniProt · HPA

UniProt accession

Q13115

Gene

DUSP4

Ensembl

ENSG00000120875

Chromosome

8

Canonical length

394 aa

Protein class

Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

394 residues, UniProt reviewed canonical sequence.

>Q13115|DUSP4
     1  MVTMEELREM DCSVLKRLMN RDENGGGAGG SGSHGTLGLP SGGKCLLLDC RPFLAHSAGY
    61  ILGSVNVRCN TIVRRRAKGS VSLEQILPAE EEVRARLRSG LYSAVIVYDE RSPRAESLRE
   121  DSTVSLVVQA LRRNAERTDI CLLKGGYERF SSEYPEFCSK TKALAAIPPP VPPSATEPLD
   181  LGCSSCGTPL HDQGGPVEIL PFLYLGSAYH AARRDMLDAL GITALLNVSS DCPNHFEGHY
   241  QYKCIPVEDN HKADISSWFM EAIEYIDAVK DCRGRVLVHC QAGISRSATI CLAYLMMKKR
   301  VRLEEAFEFV KQRRSIISPN FSFMGQLLQF ESQVLATSCA AEAASPSGPL RERGKTPATP
   361  TSQFVFSFPV SVGVHSAPSS LPYLHSPITT SPSC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DUSP4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

31 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DUSP4.

Disease | ImmuneIEDB

Conditions an epitope on DUSP4 was assayed in.

• melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.42

gnomAD pLI

0.88

gnomAD missense Z

1.49

DepMap mean gene effect

-0.08

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• dephosphorylation
• endoderm formation
• negative regulation of ERK1 and ERK2 cascade
• negative regulation of MAPK cascade
• signal transduction

Molecular functions

• MAP kinase tyrosine/serine/threonine phosphatase activity
• phosphatase activity
• phosphoprotein phosphatase activity
• protein tyrosine phosphatase activity
• protein tyrosine/threonine phosphatase activity
• MAP kinase serine/threonine phosphatase activity

Cellular components

• cytoplasm
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Dual specificity phosphatase, catalytic domain
• Tyrosine-specific protein phosphatases domain
• Rhodanese-like domain
• Protein-tyrosine phosphatase, catalytic
• Mitogen-activated protein (MAP) kinase phosphatase
• Protein-tyrosine phosphatase, active site
• Dual specificity protein phosphatase domain
• Protein-tyrosine phosphatase-like
• Rhodanese-like domain superfamily
• Rhodanese-like domain
• Dual specificity phosphatase, catalytic domain

KeywordsUniProt

• Acetylation
• Hydrolase
• Nucleus
• Phosphoprotein
• Protein phosphatase

InteractionsUniProt · HPA

Protein binding partners of DUSP4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MAPK1
• MAPK9