Seroatlas · Human Serome Atlas

DPH3

Diphthamide biosynthesis protein 3

Also known as: DELGIP, DELGIP1, DESR1, DPH3_HUMAN, DPH3A, KTI11, MGC20197, ZCSL2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96FX2
Gene: DPH3
Ensembl: ENSG00000154813
Chromosome: 3
Canonical length: 82 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a CSL zinc finger-containing protein that is required for dipthamide biosynthesis. The encoded protein is necessary for the initial step in the modification of a histidine residue in elongation factor-2 to diphthamide. This modified residue is a target for ADP ribosylation by the bacterial toxins diphtheria toxin and Pseudomonas exotoxin A. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Feb 2009]

Canonical amino-acid sequenceUniProt

82 residues, UniProt reviewed canonical sequence.

>Q96FX2|DPH3
     1  MAVFHDEVEI EDFQYDEDSE TYFYPCPCGD NFSITKEDLE NGEDVATCPS CSLIIKVIYD
    61  KDQFVCGETV PAPSANKELV KC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DPH3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 26 nTPM
tongue: 19 nTPM
liver: 14 nTPM
smooth muscle: 14 nTPM
midbrain: 14 nTPM
adipose tissue: 13 nTPM

Single-cell type

esophageal apical cells: 180 nCPM
hofbauer cells: 149 nCPM
late primary spermatocytes: 115 nCPM
early spermatids: 114 nCPM
extravillous trophoblasts: 103 nCPM
esophageal suprabasal cells: 99 nCPM

Immune cell

neutrophil: 36 nTPM
basophil: 27 nTPM
eosinophil: 26 nTPM
intermediate monocyte: 23 nTPM
non-classical monocyte: 22 nTPM
classical monocyte: 17 nTPM

Brain region

hypothalamus: 20 nTPM
medulla oblongata: 19 nTPM
white matter: 19 nTPM
pons: 18 nTPM
spinal cord: 18 nTPM
midbrain: 17 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.52
gnomAD pLI: 0.07
gnomAD missense Z: -0.09
DepMap mean gene effect: -0.9
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DPH3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

SERGEF

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DPH3 as an antibody target. Whether an autoantibody or antibody against DPH3 could matter depends on whether native DPH3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DPH3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DPH3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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