Seroatlas · Human Serome Atlas

DOLK

Dolichol kinase

Also known as: DK1, DOLK_HUMAN, KIAA1094, TMEM15

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UPQ8
Gene
DOLK
Ensembl
ENSG00000175283
Chromosome
9
Canonical length
538 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted membrane proteins
Subcellular location
Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

The protein encoded by this gene catalyzes the CTP-mediated phosphorylation of dolichol, and is involved in the synthesis of Dol-P-Man, which is an essential glycosyl carrier lipid for C- and O-mannosylation, N- and O-linked glycosylation of proteins, and for the biosynthesis of glycosyl phosphatidylinositol anchors in endoplasmic reticulum. Mutations in this gene are associated with dolichol kinase deficiency.[provided by RefSeq, Apr 2010]

Canonical amino-acid sequenceUniProt

538 residues, UniProt reviewed canonical sequence.

>Q9UPQ8|DOLK
     1  MTRECPSPAP GPGAPLSGSV LAEAAVVFAV VLSIHATVWD RYSWCAVALA VQAFYVQYKW
    61  DRLLQQGSAV FQFRMSANSG LLPASMVMPL LGLVMKERCQ TAGNPFFERF GIVVAATGMA
   121  VALFSSVLAL GITRPVPTNT CVILGLAGGV IIYIMKHSLS VGEVIEVLEV LLIFVYLNMI
   181  LLYLLPRCFT PGEALLVLGG ISFVLNQLIK RSLTLVESQG DPVDFFLLVV VVGMVLMGIF
   241  FSTLFVFMDS GTWASSIFFH LMTCVLSLGV VLPWLHRLIR RNPLLWLLQF LFQTDTRIYL
   301  LAYWSLLATL ACLVVLYQNA KRSSSESKKH QAPTIARKYF HLIVVATYIP GIIFDRPLLY
   361  VAATVCLAVF IFLEYVRYFR IKPLGHTLRS FLSLFLDERD SGPLILTHIY LLLGMSLPIW
   421  LIPRPCTQKG SLGGARALVP YAGVLAVGVG DTVASIFGST MGEIRWPGTK KTFEGTMTSI
   481  FAQIISVALI LIFDSGVDLN YSYAWILGSI STVSLLEAYT TQIDNLLLPL YLLILLMA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DOLK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
15
Mean surface accessibility (rSASA)
0.24
Highest tissue expression
16 nTPM

Expression across tissuesHPA

Tissue

  • parathyroid gland: 16 nTPM
  • testis: 14 nTPM
  • pancreas: 14 nTPM
  • adrenal gland: 14 nTPM
  • choroid plexus: 13 nTPM
  • epididymis: 13 nTPM

Single-cell type

  • late primary spermatocytes: 33 nCPM
  • cytotrophoblasts: 31 nCPM
  • early primary spermatocytes: 23 nCPM
  • syncytiotrophoblasts: 22 nCPM
  • decidual stromal cells: 21 nCPM
  • migrating cytotrophoblasts: 21 nCPM

Immune cell

  • non-classical monocyte: 21 nTPM
  • T-reg: 17 nTPM
  • myeloid DC: 17 nTPM
  • classical monocyte: 15 nTPM
  • intermediate monocyte: 15 nTPM
  • naive CD8 T-cell: 13 nTPM

Brain region

  • choroid plexus: 16 nTPM
  • midbrain: 14 nTPM
  • thalamus: 14 nTPM
  • hypothalamus: 13 nTPM
  • pons: 13 nTPM
  • medulla oblongata: 12 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DOLK.

Disease | AllUniProt

Conditions DOLK is implicated in, by any mechanism.

  • Congenital disorder of glycosylation 1M (CDG1M) MIM:610768

Disease | GeneticClinVar

14 pathogenic / likely-pathogenic of 598 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.9
gnomAD pLI
0
gnomAD missense Z
0.98
DepMap mean gene effect
-0.45
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

  • dolichol kinase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Polyprenol kinase family

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DOLK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DOLK as an antibody target. Whether an autoantibody or antibody against DOLK could matter depends on whether native DOLK is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DOLK is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DOLK as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DOLK. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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