Seroatlas · Human Serome Atlas

DOK1

Docking protein 1

Also known as: DOK1_HUMAN, p62dok

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q99704
Gene
DOK1
Ensembl
ENSG00000115325
Chromosome
2
Canonical length
481 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Canonical amino-acid sequenceUniProt

481 residues, UniProt reviewed canonical sequence.

>Q99704|DOK1
     1  MDGAVMEGPL FLQSQRFGTK RWRKTWAVLY PASPHGVARL EFFDHKGSSS GGGRGSSRRL
    61  DCKVIRLAEC VSVAPVTVET PPEPGATAFR LDTAQRSHLL AADAPSSAAW VQTLCRNAFP
   121  KGSWTLAPTD NPPKLSALEM LENSLYSPTW EGSQFWVTVQ RTEAAERCGL HGSYVLRVEA
   181  ERLTLLTVGA QSQILEPLLS WPYTLLRRYG RDKVMFSFEA GRRCPSGPGT FTFQTAQGND
   241  IFQAVETAIH RQKAQGKAGQ GHDVLRADSH EGEVAEGKLP SPPGPQELLD SPPALYAEPL
   301  DSLRIAPCPS QDSLYSDPLD STSAQAGEGV QRKKPLYWDL YEHAQQQLLK AKLTDPKEDP
   361  IYDEPEGLAP VPPQGLYDLP REPKDAWWCQ ARVKEEGYEL PYNPATDDYA VPPPRSTKPL
   421  LAPKPQGPAF PEPGTATGSG IKSHNSALYS QVQKSGASGS WDCGLSRVGT DKTGVKSEGS
   481  T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DOK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.53
Highest tissue expression
19 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 19 nTPM
  • bone marrow: 13 nTPM
  • adipose tissue: 12 nTPM
  • lymph node: 12 nTPM
  • appendix: 9.6 nTPM
  • tonsil: 9.5 nTPM

Single-cell type

  • megakaryocytes: 148 nCPM
  • platelets: 87 nCPM
  • hofbauer cells: 63 nCPM
  • megakaryocyte progenitors: 35 nCPM
  • microglia: 33 nCPM
  • hepatic stellate cells: 32 nCPM

Immune cell

  • non-classical monocyte: 60 nTPM
  • neutrophil: 53 nTPM
  • classical monocyte: 52 nTPM
  • intermediate monocyte: 51 nTPM
  • myeloid DC: 51 nTPM
  • total PBMC: 40 nTPM

Brain region

  • medulla oblongata: 12 nTPM
  • thalamus: 9.2 nTPM
  • spinal cord: 8.9 nTPM
  • white matter: 8.2 nTPM
  • hypothalamus: 7.9 nTPM
  • amygdala: 7.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.64
gnomAD pLI
0.03
gnomAD missense Z
0.09
DepMap mean gene effect
-0.13
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DOK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DOK1 as an antibody target. Whether an autoantibody or antibody against DOK1 could matter depends on whether native DOK1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DOK1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DOK1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DOK1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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