DIDO1

Protein identityUniProt · HPA

UniProt accession

Q9BTC0

Gene

DIDO1

Ensembl

ENSG00000101191

Chromosome

20

Canonical length

2240 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

2240 residues, UniProt reviewed canonical sequence.

>Q9BTC0|DIDO1
     1  MDDKGDPSNE EAPKAIKPTS KEFRKTWGFR RTTIAKREGA GDAEADPLEP PPPQQQLGLS
    61  LRRSGRQPKR TERVEQFLTI ARRRGRRSMP VSLEDSGEPT SCPATDAETA SEGSVESASE
   121  TRSGPQSAST AVKERPASSE KVKGGDDHDD TSDSDSDGLT LKELQNRLRR KREQEPTERP
   181  LKGIQSRLRK KRREEGPAET VGSEASDTVE GVLPSKQEPE NDQGVVSQAG KDDRESKLEG
   241  KAAQDIKDEE PGDLGRPKPE CEGYDPNALY CICRQPHNNR FMICCDRCEE WFHGDCVGIS
   301  EARGRLLERN GEDYICPNCT ILQVQDETHS ETADQQEAKW RPGDADGTDC TSIGTIEQKS
   361  SEDQGIKGRI EKAANPSGKK KLKIFQPVIE APGASKCIGP GCCHVAQPDS VYCSNDCILK
   421  HAAATMKFLS SGKEQKPKPK EKMKMKPEKP SLPKCGAQAG IKISSVHKRP APEKKETTVK
   481  KAVVVPARSE ALGKEAACES STPSWASDHN YNAVKPEKTA APSPSLLYKS TKEDRRSEEK
   541  AAAMAASKKT APPGSAVGKQ PAPRNLVPKK SSFANVAAAT PAIKKPPSGF KGTIPKRPWL
   601  SATPSSGASA ARQAGPAPAA ATAASKKFPG SAALVGAVRK PVVPSVPMAS PAPGRLGAMS
   661  AAPSQPNSQI RQNIRRSLKE ILWKRVNDSD DLIMTENEVG KIALHIEKEM FNLFQVTDNR
   721  YKSKYRSIMF NLKDPKNQGL FHRVLREEIS LAKLVRLKPE ELVSKELSTW KERPARSVME
   781  SRTKLHNESK KTAPRQEAIP DLEDSPPVSD SEEQQESARA VPEKSTAPLL DVFSSMLKDT
   841  TSQHRAHLFD LNCKICTGQV PSAEDEPAPK KQKLSASVKK EDLKSKHDSS APDPAPDSAD
   901  EVMPEAVPEV ASEPGLESAS HPNVDRTYFP GPPGDGHPEP SPLEDLSPCP ASCGSGVVTT
   961  VTVSGRDPRT APSSSCTAVA SAASRPDSTH MVEARQDVPK PVLTSVMVPK SILAKPSSSP
  1021  DPRYLSVPPS PNISTSESRS PPEGDTTLFL SRLSTIWKGF INMQSVAKFV TKAYPVSGCF
  1081  DYLSEDLPDT IHIGGRIAPK TVWDYVGKLK SSVSKELCLI RFHPATEEEE VAYISLYSYF
  1141  SSRGRFGVVA NNNRHVKDLY LIPLSAQDPV PSKLLPFEGP GLESPRPNII LGLVICQKIK
  1201  RPANSGELDK MDEKRTRLQP EEADVPAYPK VATVPQSEKK PSKYPLCSAD AAVSTTPPGS
  1261  PPPPPPLPEP PVLKVLSSLK PAAPSPATAA TTAAAASTAA SSTASSASKT ASPLEHILQT
  1321  LFGKKKSFDP SAREPPGSTA GLPQEPKTTA EDGVPAPPLL DPIVQQFGQF SKDKALEEEE
  1381  DDRPYDPEEE YDPERAFDTQ LVERGRRHEV ERAPEAAAAE REEVAYDPED ETILEEAKVT
  1441  VDDLPNRMCA DVRRNSVERP AEPVAGAATP SLVEQQKMLE ELNKQIEEQK RQLEEQEEAL
  1501  RQQRAAVGVS MAHFSVSDAL MSPPPKSSLP KAELFQQEQQ SADKPASLPP ASQASNHRDP
  1561  RQARRLATET GEGEGEPLSR LSARGAQGAL PERDASRGGL VGQAPMPVPE EKEPASSPWA
  1621  SGEKPPAGSE QDGWKAEPGE GTRPATVGDS SARPARRVLL PTPPCGALQP GFPLQHDGER
  1681  DPFTCPGFAS QDKALGSAQY EDPRNLHSAG RSSSPAGETE GDREPQARPG EGTAPLPPPG
  1741  QKVGGSQPPF QGQREPGPHA LGMSGLHGPN FPGPRGPAPP FPEENIASND GPRGPPPARF
  1801  GAQKGPIPSL FSGQHGPPPY GDSRGPSPSY LGGPRGVAPS QFEERKDPHG EKREFQDAPY
  1861  NEVTGAPAQF EGTEQAPFLG SRGGAPFQFG GQRRPLLSQL KGPRGGPPPS QFGGQRGPPP
  1921  GHFVGPRGPH PSQFETARGP HPNQFEGPRG QAPNFMPGPR GIQPQQFEDQ RVHSPPRFTN
  1981  QRAPAPLQFG GLRGSAPFSE KNEQTPSRFH FQGQAPQVMK PGPRPLLELP SHPPQHRKDR
  2041  WEEAGPPSAL SSSAPGQGPE ADGQWASADF REGKGHEYRN QTFEGRQRER FDVGPKEKPL
  2101  EEPDAQGRAS EDRRRERERG RNWSRERDWD RPREWDRHRD KDSSRDWDRN RERSANRDRE
  2161  READRGKEWD RSRERSRNRE RERDRRRDRD RSRSRERDRD KARDRERGRD RKDRSKSKES
  2221  ARDPKPEASR ASDAGTASQA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DIDO1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.57

Highest tissue expression

38 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.19

gnomAD pLI

1

gnomAD missense Z

1.1

DepMap mean gene effect

-0.41

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic signaling pathway
• DNA-templated transcription
• regulation of transcription by RNA polymerase II

Molecular functions

• RNA binding
• zinc ion binding

Cellular components

• cytoplasm
• nucleus
• spindle

Protein domainsUniProt · Pfam · InterPro

• Zinc finger, PHD-type
• Transcription elongation factor S-II, central domain
• Zinc finger, FYVE/PHD-type
• Spen paralogue and orthologue SPOC, C-terminal
• Zinc finger, RING/FYVE/PHD-type
• Zinc finger, PHD-type, conserved site
• Zinc finger, PHD-finger
• Transcription elongation factor S-II, central domain superfamily
• PHD-finger
• Transcription factor S-II (TFIIS), central domain
• SPOC domain
• Death-inducer obliterator 1, PHD finger

KeywordsUniProt

• Acetylation
• Apoptosis
• Cytoplasm
• Cytoskeleton
• Isopeptide bond
• Metal-binding
• Methylation
• Nucleus
• Phosphoprotein
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of DIDO1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HCFC1
• OGT