DGKE

Protein identityUniProt · HPA

UniProt accession

P52429

Gene

DGKE

Ensembl

ENSG00000153933

Chromosome

17

Canonical length

567 aa

Protein class

Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

567 residues, UniProt reviewed canonical sequence.

>P52429|DGKE
     1  MEAERRPAPG SPSEGLFADG HLILWTLCSV LLPVFITFWC SLQRSRRQLH RRDIFRKSKH
    61  GWRDTDLFSQ PTYCCVCAQH ILQGAFCDCC GLRVDEGCLR KADKRFQCKE IMLKNDTKVL
   121  DAMPHHWIRG NVPLCSYCMV CKQQCGCQPK LCDYRCIWCQ KTVHDECMKN SLKNEKCDFG
   181  EFKNLIIPPS YLTSINQMRK DKKTDYEVLA SKLGKQWTPL IILANSRSGT NMGEGLLGEF
   241  RILLNPVQVF DVTKTPPIKA LQLCTLLPYY SARVLVCGGD GTVGWVLDAV DDMKIKGQEK
   301  YIPQVAVLPL GTGNDLSNTL GWGTGYAGEI PVAQVLRNVM EADGIKLDRW KVQVTNKGYY
   361  NLRKPKEFTM NNYFSVGPDA LMALNFHAHR EKAPSLFSSR ILNKAVYLFY GTKDCLVQEC
   421  KDLNKKVELE LDGERVALPS LEGIIVLNIG YWGGGCRLWE GMGDETYPLA RHDDGLLEVV
   481  GVYGSFHCAQ IQVKLANPFR IGQAHTVRLI LKCSMMPMQV DGEPWAQGPC TVTITHKTHA
   541  MMLYFSGEQT DDDISSTSDQ EDIKATE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DGKE can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

32 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DGKE.

Disease | AllUniProt

Conditions DGKE is implicated in, by any mechanism.

• Nephrotic syndrome 7 (NPHS7) MIM:615008
• Hemolytic uremic syndrome, atypical, 7 (AHUS7) MIM:615008

Disease | GeneticClinVar

59 pathogenic / likely-pathogenic of 352 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Atypical hemolytic-uremic syndrome
• Immunoglobulin-mediated membranoproliferative glomerulonephritis
• Mesangiocapillary glomerulonephritis
• Hemolytic uremic syndrome, atypical, susceptibility to, 7
• Nephrotic syndrome

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.71

gnomAD pLI

0

gnomAD missense Z

0.59

DepMap mean gene effect

0.1

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• diacylglycerol metabolic process
• glycerolipid metabolic process
• intracellular signal transduction
• lipid phosphorylation
• modulation of chemical synaptic transmission
• phosphatidic acid biosynthetic process
• phosphatidylinositol biosynthetic process
• phospholipase C-activating G protein-coupled receptor signaling pathway
• platelet activation

Molecular functions

• ATP binding
• ATP-dependent diacylglycerol kinase activity
• kinase activity
• zinc ion binding

Cellular components

• cytoplasm
• cytosol
• glutamatergic synapse
• membrane
• nucleoplasm
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Diacylglycerol kinase, accessory domain
• Diacylglycerol kinase, catalytic domain
• Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
• NAD kinase/diacylglycerol kinase-like domain superfamily
• Inorganic polyphosphate/ATP-NAD kinase, N-terminal
• Diacylglycerol kinase
• C1-like domain superfamily
• Phorbol esters/diacylglycerol binding domain (C1 domain)
• Diacylglycerol kinase accessory domain
• Diacylglycerol kinase catalytic domain

KeywordsUniProt

• ATP-binding
• Cytoplasm
• Hemolytic uremic syndrome
• Kinase
• Lipid metabolism
• Membrane
• Metal-binding
• Nucleotide-binding
• Repeat
• Transferase
• Transmembrane
• Transmembrane helix
• Zinc
• Zinc-finger