DGKB

Protein identityUniProt · HPA

UniProt accession

Q9Y6T7

Gene

DGKB

Ensembl

ENSG00000136267

Chromosome

7

Canonical length

804 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear speckles,Plasma membrane

OverviewNCBI Gene

Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]

Canonical amino-acid sequenceUniProt

804 residues, UniProt reviewed canonical sequence.

>Q9Y6T7|DGKB
     1  MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK QDILNQTIDF
    61  EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPMVKS KPALLSGGLR MNKGAITPPR
   121  TTSPANTCSP EVIHLKDIVC YLSLLERGRP EDKLEFMFRL YDTDGNGFLD SSELENIISQ
   181  MMHVAEYLEW DVTELNPILH EMMEEIDYDH DGTVSLEEWI QGGMTTIPLL VLLGLENNVK
   241  DDGQHVWRLK HFNKPAYCNL CLNMLIGVGK QGLCCSFCKY TVHERCVARA PPSCIKTYVK
   301  SKRNTDVMHH YWVEGNCPTK CDKCHKTVKC YQGLTGLHCV WCQITLHNKC ASHLKPECDC
   361  GPLKDHILPP TTICPVVLQT LPTSGVSVPE ERQSTVKKEK SGSQQPNKVI DKNKMQRANS
   421  VTVDGQGLQV TPVPGTHPLL VFVNPKSGGK QGERIYRKFQ YLLNPRQVYS LSGNGPMPGL
   481  NFFRDVPDFR VLACGGDGTV GWVLDCIEKA NVGKHPPVAI LPLGTGNDLA RCLRWGGGYE
   541  GENLMKILKD IENSTEIMLD RWKFEVIPND KDEKGDPVPY SIINNYFSIG VDASIAHRFH
   601  IMREKHPEKF NSRMKNKFWY FEFGTSETFS ATCKKLHESV EIECDGVQID LINISLEGIA
   661  ILNIPSMHGG SNLWGESKKR RSHRRIEKKG SDKRTTVTDA KELKFASQDL SDQLLEVVGL
   721  EGAMEMGQIY TGLKSAGRRL AQCSCVVIRT SKSLPMQIDG EPWMQTPCTI KITHKNQAPM
   781  LMGPPPKTGL FCSLVKRTRN RSKE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DGKB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

19 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.37

gnomAD pLI

0.53

gnomAD missense Z

1.95

DepMap mean gene effect

0.08

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• diacylglycerol metabolic process
• glycerolipid metabolic process
• intracellular signal transduction
• lipid phosphorylation
• phosphatidic acid biosynthetic process
• phospholipase C-activating G protein-coupled receptor signaling pathway
• platelet activation

Molecular functions

• ATP binding
• ATP-dependent diacylglycerol kinase activity
• calcium ion binding
• lipid binding
• zinc ion binding

Cellular components

• cytoplasm
• cytosol
• nuclear speck
• nucleoplasm
• plasma membrane
• postsynaptic membrane

Protein domainsUniProt · Pfam · InterPro

• Diacylglycerol kinase, accessory domain
• Diacylglycerol kinase, catalytic domain
• EF-hand domain
• Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
• EF-hand domain pair
• NAD kinase/diacylglycerol kinase-like domain superfamily
• Inorganic polyphosphate/ATP-NAD kinase, N-terminal
• EF-Hand 1, calcium-binding site
• Diacylglycerol kinase type I, N-terminal
• Diacylglycerol kinase
• DGK type I, N-terminal domain superfamily
• C1-like domain superfamily
• Phorbol esters/diacylglycerol binding domain (C1 domain)
• Diacylglycerol kinase accessory domain
• Diacylglycerol kinase catalytic domain
• EF-hand domain pair
• Diacylglycerol kinase N-terminus
• Diacylglycerol kinase beta, second protein kinase C conserved region 1
• Diacylglycerol kinase beta-like, first protein kinase C conserved region 1

KeywordsUniProt

• ATP-binding
• Calcium
• Cell membrane
• Cytoplasm
• Kinase
• Lipid metabolism
• Membrane
• Metal-binding
• Nucleotide-binding
• Phosphoprotein
• Postsynaptic cell membrane
• Repeat
• Synapse
• Transferase
• Zinc
• Zinc-finger