Seroatlas · Human Serome Atlas

DGKA

Diacylglycerol kinase alpha

Also known as: DAGK, DAGK1, DGK-alpha, DGKA_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P23743
Gene
DGKA
Ensembl
ENSG00000065357
Chromosome
12
Canonical length
735 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles,Mitochondria

OverviewNCBI Gene

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]

Canonical amino-acid sequenceUniProt

735 residues, UniProt reviewed canonical sequence.

>P23743|DGKA
     1  MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA KYVQGDAIGY EGFQQFLKIY
    61  LEVDNVPRHL SLALFQSFET GHCLNETNVT KDVVCLNDVS CYFSLLEGGR PEDKLEFTFK
   121  LYDTDRNGIL DSSEVDKIIL QMMRVAEYLD WDVSELRPIL QEMMKEIDYD GSGSVSQAEW
   181  VRAGATTVPL LVLLGLEMTL KDDGQHMWRP KRFPRPVYCN LCESSIGLGK QGLSCNLCKY
   241  TVHDQCAMKA LPCEVSTYAK SRKDIGVQSH VWVRGGCESG RCDRCQKKIR IYHSLTGLHC
   301  VWCHLEIHDD CLQAVGHECD CGLLRDHILP PSSIYPSVLA SGPDRKNSKT SQKTMDDLNL
   361  STSEALRIDP VPNTHPLLVF VNPKSGGKQG QRVLWKFQYI LNPRQVFNLL KDGPEIGLRL
   421  FKDVPDSRIL VCGGDGTVGW ILETIDKANL PVLPPVAVLP LGTGNDLARC LRWGGGYEGQ
   481  NLAKILKDLE MSKVVHMDRW SVEVIPQQTE EKSDPVPFQI INNYFSIGVD ASIAHRFHIM
   541  REKYPEKFNS RMKNKLWYFE FATSESIFST CKKLEESLTV EICGKPLDLS NLSLEGIAVL
   601  NIPSMHGGSN LWGDTRRPHG DIYGINQALG ATAKVITDPD ILKTCVPDLS DKRLEVVGLE
   661  GAIEMGQIYT KLKNAGRRLA KCSEITFHTT KTLPMQIDGE PWMQTPCTIK ITHKNQMPML
   721  MGPPPRSTNF FGFLS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DGKA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
185 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 185 nTPM
  • tonsil: 95 nTPM
  • esophagus: 92 nTPM
  • lymph node: 89 nTPM
  • skin: 80 nTPM
  • small intestine: 67 nTPM

Single-cell type

  • esophageal apical cells: 200 nCPM
  • enterocytes: 97 nCPM
  • ocular epithelial cells: 88 nCPM
  • prostatic hillock cells: 85 nCPM
  • thymocytes: 75 nCPM
  • t-cells: 69 nCPM

Immune cell

  • naive CD4 T-cell: 87 nTPM
  • T-reg: 87 nTPM
  • memory CD4 T-cell: 71 nTPM
  • naive CD8 T-cell: 52 nTPM
  • total PBMC: 36 nTPM
  • MAIT T-cell: 36 nTPM

Brain region

  • basal ganglia: 19 nTPM
  • cerebral cortex: 16 nTPM
  • amygdala: 14 nTPM
  • choroid plexus: 13 nTPM
  • hippocampal formation: 13 nTPM
  • cerebellum: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.37
gnomAD pLI
0.46
gnomAD missense Z
2.5
DepMap mean gene effect
-0.11
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DGKA as an antibody target. Whether an autoantibody or antibody against DGKA could matter depends on whether native DGKA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DGKA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DGKA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DGKA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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