Seroatlas · Human Serome Atlas

DENND6A

Protein DENND6A

Also known as: AFI1A, DEN6A_HUMAN, FAM116A, FLJ34969

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8IWF6
Gene
DENND6A
Ensembl
ENSG00000174839
Chromosome
3
Canonical length
608 aa
Protein class
Predicted intracellular proteins
Subcellular location
Vesicles

OverviewNCBI Gene

Enables guanyl-nucleotide exchange factor activity. Involved in positive regulation of cell-cell adhesion mediated by cadherin. Located in recycling endosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

608 residues, UniProt reviewed canonical sequence.

>Q8IWF6|DENND6A
     1  MALRGPAGLG PGSRRPLDEA VAGAEGREAP ALVAAGGAPE DDEEDDGRGR GLLRWDSFSA
    61  WLHCVCVVGF DLELGQAVEV IYPQHSKLTD REKTNICYLS FPDSNSGCLG DTQFCFRFRQ
   121  SSGRRVSLHC LLDQFDKDLP VYLKKDPAYF YGYVYFRQVR DKTLKRGYFQ KSLVLISKLP
   181  YIHFFHTVLK QIAPEYFEKN EPYLEAACND VDRWPAPVPG KTLHLPIMGV VMKVRIPTCH
   241  DKPGTTQIVQ LTQQVDTNIS VILPTVHEVD IFRCFCPVFL HSQMLWELVL LGEPLVVMAP
   301  SPSESSETVL ALVNCISPLK YFSDFRPYFT IHDSEFKEYT TRTQAPPSVI LGVTNPFFAK
   361  TLQHWPHIIR IGDLKPTGEI PKQVKVKKLK NLKTLDSKPG VYTSYKPYLN RDEEIIKQLQ
   421  KGVQQKRPSE AQSVILRRYF LELTQSFIIP LERYVASLMP LQKSISPWKS PPQLRQFLPE
   481  EFMKTLEKTG PQLTSRIKGD WIGLYRHFLK SPNFDGWFKT RRKEMTQKLE ALHLEALCEE
   541  DLLLWIQKHT EVETVDLVLK LKNKLLQADR EHLPVKPDTM EKLRTHIDAI ILALPEDLQG
   601  ILLKTGMT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DENND6A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
15 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 15 nTPM
  • thyroid gland: 13 nTPM
  • spleen: 12 nTPM
  • blood vessel: 12 nTPM
  • breast: 12 nTPM
  • endometrium: 12 nTPM

Single-cell type

  • cardiomyocytes: 251 nCPM
  • neutrophils: 179 nCPM
  • pituitary stem cells: 168 nCPM
  • medullary thymic epithelial cells: 151 nCPM
  • neutrophil progenitors: 147 nCPM
  • fibro-adipogenic progenitors: 142 nCPM

Immune cell

  • basophil: 19 nTPM
  • naive CD4 T-cell: 3.4 nTPM
  • MAIT T-cell: 2.9 nTPM
  • naive CD8 T-cell: 2.6 nTPM
  • gdT-cell: 2.4 nTPM
  • memory CD8 T-cell: 2.4 nTPM

Brain region

  • cerebellum: 41 nTPM
  • white matter: 31 nTPM
  • cerebral cortex: 26 nTPM
  • pons: 25 nTPM
  • hypothalamus: 25 nTPM
  • medulla oblongata: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.49
gnomAD pLI
0
gnomAD missense Z
2.07
DepMap mean gene effect
0.14
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DENND6A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DENND6A as an antibody target. Whether an autoantibody or antibody against DENND6A could matter depends on whether native DENND6A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DENND6A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DENND6A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DENND6A. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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