Seroatlas · Human Serome Atlas

DENND4B

DENN domain-containing protein 4B

Also known as: DEN4B_HUMAN, KIAA0476

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O75064
Gene: DENND4B
Ensembl: ENSG00000198837
Chromosome: 1
Canonical length: 1496 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Golgi apparatus

OverviewNCBI Gene

Enables guanyl-nucleotide exchange factor activity. Involved in regulation of Rab protein signal transduction. Located in Golgi apparatus and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1496 residues, UniProt reviewed canonical sequence.

>O75064|DENND4B
     1  MAEERPPRLV DYFVVAGLAG NGAPIPEETW VPEPSGPLRP PRPAEPITDV AVIARALGEE
    61  VPQGYTCIQA SAGGHPLELS AGLLGGTQPV ICYRRGRDKP PLVELGVLYE GKERPKPGFQ
   121  VLDTTPYSHS ANLAPPGPGH PRTYLTYRRA AEGAGLHALG ITDLCLVLPS KGEGTPHTYC
   181  RLPRNLNPGM WGPAVYLCYK VGLAKANTLV YEAELLGRYP EEDNEAFPLP ESVPVFCLPM
   241  GATIECWPAQ TKYPVPVFST FVLTGAAGDK VYGAALQFYE AFPRARLSER QARALGLLSA
   301  VERGRALGGR AVRSRRAIAV LSRWPAFPAF RAFLTFLYRY SVSGPHRLPL EAHISHFIHN
   361  VPFPSPQRPR ILVQMSPYDN LLLCQPVSSP LPLSGASFLQ LLQSLGPELA ITLLLAVLTE
   421  HKLLVHSLRP DLLTSVCEAL VSMIFPLHWQ CPYIPLCPLV LADVLSAPVP FIVGIHSSYF
   481  DLHDPPADVI CVDLDTNTLF QTEEKKLLSP RTLPRRPYKV LLATLTNLYQ QLDQTYTGPE
   541  EEASLEFLLT DYEAVCGRRA RLEREVQGAF LRFMACLLKG YRVFLRPLTQ APSEGARDVD
   601  NLFFLQGFLK SRERSSHKLY SQLLHTQMFS QFIEECSFGS ARHAALEFFD SCVEKVHPEQ
   661  EKPEPTPLVE LEELSGSELT VFITPPEEPA LPEGSESTPQ YCYDGFPELR AELFESLQEQ
   721  PGALPVPGPS RSAPSSPAPR RTKQEMKVAQ RMAQKSAAVP ELWARCLLGH CYGLWFLCLP
   781  AYVRSAPSRV QALHTAYHVL RQMESGKVVL PDEVCYRVLM QLCSHYGQPV LSVRVMLEMR
   841  QAGIVPNTIT YGYYNKAVLE SKWPSGTPGG RLRWAKLRNV VLGAAQFRQP LRERQQQQQQ
   901  QQQQQQQQQQ EQVSAHQEAG SSQAEPYLER PSPTRPLQRQ TTWAGRSLRD PASPPGRLVK
   961  SGSLGSARGA QPTVEAGVAH MIEALGVLEP RGSPVPWHDG SLSDLSLTGE EPLPGGSPGG
  1021  SGSALSAQST EALEGLSGRG PKAGGRQDEA GTPRRGLGAR LQQLLTPSRH SPASRIPPPE
  1081  LPPDLPPPAR RSPMDSLLHP RERPGSTASE SSASLGSEWD LSESSLSNLS LRRSSERLSD
  1141  TPGSFQSPSL EILLSSCSLC RACDSLVYDE EIMAGWAPDD SNLNTTCPFC ACPFVPLLSV
  1201  QTLDSRPSVP SPKSAGASGS KDAPVPGGPG PVLSDRRLCL ALDEPQLCNG HMGGASRRVE
  1261  SGAWAYLSPL VLRKELESLV ENEGSEVLAL PELPSAHPII FWNLLWYFQR LRLPSILPGL
  1321  VLASCDGPSH SQAPSPWLTP DPASVQVRLL WDVLTPDPNS CPPLYVLWRV HSQIPQRVVW
  1381  PGPVPASLSL ALLESVLRHV GLNEVHKAVG LLLETLGPPP TGLHLQRGIY REILFLTMAA
  1441  LGKDHVDIVA FDKKYKSAFN KLASSMGKEE LRHRRAQMPT PKAIDCRKCF GAPPEC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DENND4B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 46 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 46 nTPM
spleen: 39 nTPM
bone marrow: 35 nTPM
cerebellum: 34 nTPM
cerebral cortex: 30 nTPM
lymph node: 28 nTPM

Single-cell type

microglia: 65 nCPM
neutrophils: 62 nCPM
monocytes: 58 nCPM
pdcs: 58 nCPM
macrophages: 54 nCPM
kupffer cells: 51 nCPM

Immune cell

intermediate monocyte: 2.4 nTPM
non-classical monocyte: 1.9 nTPM
plasmacytoid DC: 1.9 nTPM
classical monocyte: 1.6 nTPM
basophil: 1.5 nTPM
neutrophil: 1.5 nTPM

Brain region

cerebral cortex: 74 nTPM
pons: 62 nTPM
amygdala: 58 nTPM
medulla oblongata: 56 nTPM
thalamus: 56 nTPM
white matter: 54 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DENND4B.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 242 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Short stature

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.29
gnomAD pLI: 0.99
gnomAD missense Z: 3.23
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

regulation of Rab protein signal transduction

Molecular functions

guanyl-nucleotide exchange factor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DENND4B as an antibody target. Whether an autoantibody or antibody against DENND4B could matter depends on whether native DENND4B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DENND4B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DENND4B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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