DENND2B

Protein identityUniProt · HPA

UniProt accession

P78524

Gene

DENND2B

Ensembl

ENSG00000166444

Chromosome

11

Canonical length

1137 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1137 residues, UniProt reviewed canonical sequence.

>P78524|DENND2B
     1  MTMTANKNSS ITHGAGGTKA PRGTLSRSQS VSPPPVLSPP RSPIYPLSDS ETSACRYPSH
    61  SSSRVLLKDR HPPAPSPQNP QDPSPDTSPP TCPFKTASFG YLDRSPSACK RDAQKESVQG
   121  AAQDVAGVAA CLPLAQSTPF PGPAAGPRGV LLTRTGTRAH SLGIREKISA WEGRREASPR
   181  MSMCGEKREG SGSEWAASEG CPSLGCPSVV PSPCSSEKTF DFKGLRRMSR TFSECSYPET
   241  EEEGEALPVR DSFYRLEKRL GRSEPSAFLR GHGSRKESSA VLSRIQKIEQ VLKEQPGRGL
   301  PQLPSSCYSV DRGKRKTGTL GSLEEPAGGA SVSAGSRAVG VAGVAGEAGP PPEREGSGST
   361  KPGTPGNSPS SQRLPSKSSL DPAVNPVPKP KRTFEYEADK NPKSKPSNGL PPSPTPAAPP
   421  PLPSTPAPPV TRRPKKDMRG HRKSQSRKSF EFEDASSLQS LYPSSPTENG TENQPKFGSK
   481  STLEENAYED IVGDLPKENP YEDVDLKSRR AGRKSQQLSE NSLDSLHRMW SPQDRKYNSP
   541  PTQLSLKPNS QSLRSGNWSE RKSHRLPRLP KRHSHDDMLL LAQLSLPSSP SSLNEDSLST
   601  TSELLSSRRA RRIPKLVQRI NSIYNAKRGK KRLKKLSMSS IETASLRDEN SESESDSDDR
   661  FKAHTQRLVH IQSMLKRAPS YRTLELELLE WQERELFEYF VVVSLKKKPS RNTYLPEVSY
   721  QFPKLDRPTK QMREAEERLK AIPQFCFPDA KDWLPVSEYS SETFSFMLTG EDGSRRFGYC
   781  RRLLPSGKGP RLPEVYCVIS RLGCFGLFSK VLDEVERRRG ISAALVYPFM RSLMESPFPA
   841  PGKTIKVKTF LPGAGNEVLE LRRPMDSRLE HVDFECLFTC LSVRQLIRIF ASLLLERRVI
   901  FVADKLSTLS SCSHAVVALL YPFSWQHTFI PVLPASMIDI VCCPTPFLVG LLSSSLPKLK
   961  ELPVEEALMV NLGSDRFIRQ MDDEDTLLPR KLQAALEQAL ERKNELISQD SDSDSDDECN
  1021  TLNGLVSEVF IRFFVETVGH YSLFLTQSEK GERAFQREAF RKSVASKSIR RFLEVFMESQ
  1081  MFAGFIQDRE LRKCRAKGLF EQRVEQYLEE LPDTEQSGMN KFLRGLGNKM KFLHKKN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DENND2B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.52

Highest tissue expression

128 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.21

gnomAD pLI

1

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• positive regulation of ERK1 and ERK2 cascade

Molecular functions

• guanyl-nucleotide exchange factor activity

Cellular components

• cell cortex
• plasma membrane
• recycling endosome

Protein domainsUniProt · Pfam · InterPro

• cDENN domain
• dDENN domain
• uDENN domain
• Tripartite DENN domain
• DENN domain, C-terminal lobe
• DENN domain-containing protein 2
• DENN (AEX-3) domain
• dDENN domain
• uDENN domain

KeywordsUniProt

• Alternative promoter usage
• Cell membrane
• Cytoplasm
• Endosome
• Guanine-nucleotide releasing factor
• Membrane
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of DENND2B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GRB2
• ITSN1
• SFN