Seroatlas · Human Serome Atlas

DDIT4

DNA damage-inducible transcript 4 protein

Also known as: DDIT4_HUMAN, Dig2, FLJ20500, REDD-1, REDD1, RTP801

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NX09
Gene: DDIT4
Ensembl: ENSG00000168209
Chromosome: 10
Canonical length: 232 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

Predicted to enable 14-3-3 protein binding activity. Involved in negative regulation of TOR signaling and response to hypoxia. Located in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

232 residues, UniProt reviewed canonical sequence.

>Q9NX09|DDIT4
     1  MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS
    61  NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP
   121  SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR
   181  LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDIT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 596 nTPM

Expression across tissuesHPA

Tissue

ovary: 596 nTPM
pancreas: 480 nTPM
vagina: 455 nTPM
blood vessel: 393 nTPM
cervix: 376 nTPM
esophagus: 316 nTPM

Single-cell type

esophageal apical cells: 4,027 nCPM
esophageal suprabasal cells: 1,522 nCPM
prostatic hillock cells: 1,046 nCPM
basal prostatic cells: 903 nCPM
pancreatic acinar cells: 805 nCPM
breast lactating cells: 801 nCPM

Immune cell

NK-cell: 409 nTPM
naive CD4 T-cell: 275 nTPM
MAIT T-cell: 250 nTPM
gdT-cell: 237 nTPM
total PBMC: 215 nTPM
naive CD8 T-cell: 214 nTPM

Brain region

choroid plexus: 82 nTPM
medulla oblongata: 80 nTPM
white matter: 66 nTPM
thalamus: 65 nTPM
midbrain: 57 nTPM
cerebellum: 56 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.28
gnomAD pLI: 0
gnomAD missense Z: 0.1
DepMap mean gene effect: 0.12
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

14-3-3 protein binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DDIT4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DDIT4 as an antibody target. Whether an autoantibody or antibody against DDIT4 could matter depends on whether native DDIT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DDIT4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DDIT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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